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Engineering cyanobacteria as cell factories for direct trehalose production from CO2.
Metabolic Engineering ( IF 6.8 ) Pub Date : 2020-09-06 , DOI: 10.1016/j.ymben.2020.08.014
Yue Qiao 1 , Weihua Wang 2 , Xuefeng Lu 3
Affiliation  

Trehalose is a non-reducing disaccharide with a wide range of applications in food, cosmetic, and pharmaceutical industries. Cyanobacteria are promising cell factories to produce biochemicals by using solar energy and CO2. Trehalose is biosynthesized at low intracellular concentrations as a salt-inducible compatible solute in some cyanobacteria. In the current study, we demonstrated the efficient trehalose production without salt induction in cyanobacteria by metabolic engineering. The trehalose transporter 1 (TRET1) from an anhydrobiotic insect (Polypedilum vanderplanki) was successfully expressed in the engineered strains and the intracellular trehalose was efficiently secreted to the medium. As the results, the engineered strain co-expressing maltooligosyl trehalose synthase (MTS), maltooligosyl trehalose trehalohydrolase (MTH) and TRET1 secreted 97% of trehalose to the medium, and the titer was up to 2.7 g/L in 15 days. In addition, 5.7 g/L trehalose was produced by semi-continuous cultivation in 34 days. Taken together, this work demonstrates cyanobacteria can be applied as cell factories for direct sunlight-driven conversion of CO2 into excreted trehalose.



中文翻译:

工程蓝藻作为细胞工厂,用于从 CO2 直接生产海藻糖。

海藻糖是一种非还原性二糖,在食品、化妆品和制药行业有广泛的应用。蓝藻是很有前途的细胞工厂,可以利用太阳能和 CO 2生产生化产品。海藻糖是在低细胞内浓度下生物合成的,作为某些蓝藻中的盐诱导相容溶质。在目前的研究中,我们通过代谢工程证明了在蓝藻中没有盐诱导的有效海藻糖生产。来自无水生物昆虫 ( Polypedilum vanderplanki ) 的海藻糖转运蛋白 1 (TRET1)) 在工程菌株中成功表达,细胞内海藻糖被有效分泌到培养基中。结果表明,共表达麦芽寡糖基海藻糖合酶(MTS)、麦芽寡糖基海藻糖海藻糖水解酶(MTH)和TRET1的工程菌株向培养基分泌了97%的海藻糖,15天内滴度高达2.7 g/L。此外,半连续培养34天可生产5.7g/L海藻糖。总而言之,这项工作表明蓝藻可以用作细胞工厂,用于将 CO 2直接转化为排泄的海藻糖。

更新日期:2020-09-06
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