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Wild apple (Malus spp.) by-products as a source of phenolic compounds and vitamin C for food applications
Food Bioscience ( IF 4.8 ) Pub Date : 2020-09-06 , DOI: 10.1016/j.fbio.2020.100744
Vitalijs Radenkovs , Tõnu Püssa , Karina Juhnevica-Radenkova , Jorens Kviesis , Francisco J. Salar , Diego A. Moreno , Inese Drudze

The phytochemical profile and positive health effects of the fruits and by-products of domesticated Malus domestica Borkh. apple cultivars have been extensively investigated. The present study was designed to evaluate the hydrophilic composition and antioxidant activity of the compounds recovered from wild apple Malus spp. by-products. The phytochemical analysis of the 30 and 96% ethanolic extracts using LC-DAD-ESI-MS/MS has led to the detection and isolation of 25 hydrophilic compounds. Amongst the polyphenols, chlorogenic acid, B-type procyanidins, and phloretin-2-glucoside dominated. The results of HPLC-DPPH radical scavenging showed that the major contributors to the antioxidant activity of wild apple by-products were found to be cyanidin-glucoside, luteolin-glucoside, and B-type (−)-epicatechin, which showed the lowest EC50 values of 0.65, 0.70, and 0.80, respectively. The abundance of vitamin C, including ascorbic and dehydroascorbic acids was confirmed using UHPLC-QqQ-MS/MS technique. The results showed that the wild apple by-products have the potential to be used in the production of natural antioxidants. Using a common solid-liquid extraction technique, 100 kg of press cake yielded 200 g of procyanidins, 100 g of chlorogenic acid, 87 g of phloridzin and 60 g of vitamin C, compounds that could potentially be used for the production of lipid oxidation inhibitors and agents with antimicrobial activities as medicines and food additives.



中文翻译:

野苹果(苹果属)的副产物酚类化合物的来源和维生素C用于食品应用

驯化的苹果属苹果的果实和副产物的植物化学特征和对健康的积极影响。苹果品种已被广泛研究。本研究的目的是评估所述亲水性组合物和所述化合物的抗氧化活性与野生苹果回收苹果属。副产品。使用LC-DAD-ESI-MS / MS对30%和96%乙醇提取物进行植物化学分析已导致检测和分离25种亲水性化合物。在多酚中,绿原酸,B型原花青素和酞菁-2-葡萄糖苷占主导地位。HPLC-DPPH的结果自由基清除表明,发现野生苹果副产物抗氧化活性的主要成分是花青素-葡萄糖苷,木犀草素-葡萄糖苷和B型(-)-表儿茶素,其EC 50最低。值分别为0.65、0.70和0.80。使用UHPLC-QqQ-MS / MS技术确认了维生素C的含量,其中包括抗坏血酸和脱氢抗坏血酸。结果表明,野苹果副产物具有用于天然抗氧化剂生产的潜力。使用常见的固液萃取技术,可将100千克压滤饼产生200克原花青素,100克绿原酸,87克磷霉素和60克维生素C,这些化合物可潜在地用于生产脂质氧化抑制剂以及具有抗微生物活性的药物和食品添加剂。

更新日期:2020-09-14
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