Size-exclusion chromatography with multi-angle laser-light scattering and refractive index detection (SEC/MALLS/RI) provides the number- and weight-average molar masses, molar mass distributions, conformations, and linear/branched structures of polymers. In the case of pure celluloses including highly crystalline tunicate and alga celluloses, and hemicellulose-rich plant holocelluloses, soaking in ethylene diamine (EDA) and subsequent solvent exchange to N,N-dimethylacetamide (DMAc) though methanol is effective for complete dissolution in ∼8% (w/w) LiCl/DMAc. SEC/MALLS/RI analysis can, therefore, be applied to pure celluloses, chemical wood pulps, and plant holocelluloses after dissolution in ∼8% (w/w) LiCl/DMAc, dilution to 1% (w/v) LiCl/DMAc and membrane filtration. All pure celluloses and the high-molar-mass cellulose fractions of hardwood and grass holocelluloses have linear and random-coil conformations and various average molar masses and molar mass distributions depending on the cellulose and holocellulose resources. In contrast, Japanese cedar (i.e., softwood) holocellulose and softwood bleached kraft pulp have alkali-stable cellulose/glucomannan branched structures in the high-molar-mass fractions.

具有多角度激光散射和折射率检测的尺寸排阻色谱法（SEC / MALLS / RI）提供了聚合物的数均和重均摩尔质量，摩尔质量分布，构象以及线性/分支结构。对于纯纤维素，包括高度结晶的被膜和藻类纤维素，以及富含半纤维素的植物全纤维素，浸泡在乙二胺（EDA）中，然后将溶剂交换为N，N-二甲基乙酰胺（DMAc）通过甲醇有效地完全溶解在8％（w / w）的LiCl / DMAc中。因此，将SEC / MALLS / RI分析溶解于约8％（w / w）LiCl / DMAc，稀释至1％（w / v）LiCl / DMAc后，可应用于纯纤维素，化学木浆和植物全纤维素。和膜过滤。硬木和草皮全纤维素的所有纯纤维素以及高摩尔质量纤维素馏分均具有线性和无规卷曲构型，并且取决于纤维素和全纤维素资源的不同平均摩尔质量和摩尔质量分布。相反，日本雪松（即针叶木）全纤维素和针叶木漂白牛皮纸浆在高摩尔质量分数中具有碱稳定的纤维素/葡甘露聚糖支化结构。