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The long-term effects of Δ9-tetrahydrocannabinol on microtubule dynamicity in rats.
Archives of Biochemistry and Biophysics ( IF 3.8 ) Pub Date : 2020-09-06 , DOI: 10.1016/j.abb.2020.108574
Dariush Gholami 1 , Ali Reza Noori 2 , Mina Mohammadkhani 3 , Zeinab Emruzi 4 , Gholam Hossein Riazi 3
Affiliation  

Studies reported that Δ9-tetrahydrocannabinol (Δ9-THC) is an essential drug as an anti-cancer, neuroprotective, anti-inflammatory, and immune-modulatory agent. However, the mechanism by which Δ9-THC causes these events remains to be elucidated. We attempted to investigate the in vivo studies of Δ9-THC on brain microtubule dynamicity, and acetylcholinesterase (AChE) activity. The microtubule polymerization, secondary and tertiary structures of α/β-tubulins, as well as the AChE activity, were evaluated in the experimental groups. The significantly lowest optical density and initial rate of polymerization was observed in THC 3 mg/kg, THC 9 mg/kg, and THC 18 mg/kg treated groups. The content of secondary and tertiary structures of α/β-tubulins was significantly affected in treated groups. The AChE activity was significantly lower in treated groups in a dose-dependent manner. These data highlight the microtubule dynamicity as a molecular target for Δ9-THC, which affects memory dysfunction. However, Δ9-THC can be inhibited the AChE activity and provide an improved therapeutics for neurodegenerative diseases.



中文翻译:

Δ9-四氢大麻酚对大鼠微管动力学的长期影响。

研究报道,Δ 9 -四氢大麻酚(Δ 9 -THC)是一个重要的药物作为抗癌症,神经保护,抗炎和免疫调节剂。然而,通过该Δ的机构9 -THC导致这些事件尚待阐明。我们试图探讨Δ的体内研究9-THC对大脑微管动力学和乙酰胆碱酯酶(AChE)活性的影响。在实验组中评估了α/β-微管蛋白的微管聚合,二级和三级结构以及AChE活性。在THC 3 mg / kg,THC 9 mg / kg和THC 18 mg / kg处理组中观察到最低的光密度和初始聚合速率。α/β-微管蛋白的二级和三级结构的含量在治疗组中受到显着影响。在治疗组中,AChE活性以剂量依赖性方式显着降低。这些数据突出了微管动态性作为Δ分子靶9 -THC,从而影响记忆功能障碍。然而,Δ 9-THC可以抑制AChE活性,并为神经退行性疾病提供改进的治疗方法。

更新日期:2020-09-12
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