Studies reported that Δ⁹-tetrahydrocannabinol (Δ⁹-THC) is an essential drug as an anti-cancer, neuroprotective, anti-inflammatory, and immune-modulatory agent. However, the mechanism by which Δ⁹-THC causes these events remains to be elucidated. We attempted to investigate the in vivo studies of Δ⁹-THC on brain microtubule dynamicity, and acetylcholinesterase (AChE) activity. The microtubule polymerization, secondary and tertiary structures of α/β-tubulins, as well as the AChE activity, were evaluated in the experimental groups. The significantly lowest optical density and initial rate of polymerization was observed in THC 3 mg/kg, THC 9 mg/kg, and THC 18 mg/kg treated groups. The content of secondary and tertiary structures of α/β-tubulins was significantly affected in treated groups. The AChE activity was significantly lower in treated groups in a dose-dependent manner. These data highlight the microtubule dynamicity as a molecular target for Δ⁹-THC, which affects memory dysfunction. However, Δ⁹-THC can be inhibited the AChE activity and provide an improved therapeutics for neurodegenerative diseases.

研究报道，Δ ⁹ -四氢大麻酚（Δ ⁹ -THC）是一个重要的药物作为抗癌症，神经保护，抗炎和免疫调节剂。然而，通过该Δ的机构⁹ -THC导致这些事件尚待阐明。我们试图探讨Δ的体内研究⁹-THC对大脑微管动力学和乙酰胆碱酯酶（AChE）活性的影响。在实验组中评估了α/β-微管蛋白的微管聚合，二级和三级结构以及AChE活性。在THC 3 mg / kg，THC 9 mg / kg和THC 18 mg / kg处理组中观察到最低的光密度和初始聚合速率。α/β-微管蛋白的二级和三级结构的含量在治疗组中受到显着影响。在治疗组中，AChE活性以剂量依赖性方式显着降低。这些数据突出了微管动态性作为Δ分子靶⁹ -THC，从而影响记忆功能障碍。然而，Δ ⁹-THC可以抑制AChE活性，并为神经退行性疾病提供改进的治疗方法。