The aim of this study was to evaluate the structure of soil bacterial and fungal communities in restinga areas of the Atlantic Forest (southern Brazil) after the introduction of Pinus L. Soil samples were collected in the 0–10 cm depth from two arboreal (FR-restinga subject to flooding and DR-dry restinga) and one herbaceous (MR-restinga under marine influence) areas. In addition to these areas, soil samples were collected from FR and DR under the influence of Pinus sp. (FR_Pinus and DR_Pinus, respectively), as reference areas. The structure of bacterial and fungal communities was evaluated by PCR-DGGE. Notably, a reduction in the number of amplicons was observed in areas with presence of Pinus sp. In conclusion, the present manuscript highlighted that the allochthonous tree species (Pinus sp.) has a deleterious impact on soil microbes and affects the growth of natural vegetation in a conservation unit in the Atlantic Forest of the Southern Brazilian coast.

本研究的目的是评估引入Pinus L后大西洋森林（巴西南部）的resta地区土壤细菌和真菌群落的结构。从两个树木（FR）的0–10 cm深度收集土壤样品-雷斯廷加受到洪水和DR-干雷斯廷加）和草本一个（MR-雷斯廷加下海洋影响）的区域。除这些地区外，在松树属菌种的影响下，还从FR和DR中收集了土壤样品。（FR_松树和DR_松树，分别作为参考区域。通过PCR-DGGE评估细菌和真菌群落的结构。值得注意的是，在存在松属物种的区域中观察到扩增子数量的减少。总之，本手稿着重指出，异种树种（Pinus sp。）对土壤微生物具有有害影响，并影响巴西南部沿海大西洋森林保护区内自然植被的生长。