The objective of this study is to investigate the effects of sea buckthorn oil (SBO) on proliferation, adipogenic differentiation and insulin sensitivity of 3T3-L1 cells. Results showed that SBO increased cell proliferation ability, accompanied by up-regulated proliferating cell nuclear antigen content (p < 0.05) and p38 activity (p < 0.05). SBO also promoted adipogenesis and enhanced adipogenic transcriptional factors expression. Mitochondrial biogenesis related gene expressions were elevated in SBO treated cells (p < 0.05). Of note, SBO also increased glucose uptake and glucose transporter 4 abundance (p < 0.05). Cells treated with SBO exhibited greater phosphorylated insulin receptor substrate 1 (p < 0.05), phosphorylated-Akt (p < 0.05) and phosphorylated AMP-activated protein kinase (p < 0.01) contents. When taken together, these results suggest that SBO promotes 3T3-L1 cells proliferation, adipogenesis and insulin sensitivity.

本研究的目的是研究沙棘油 (SBO) 对 3T3-L1 细胞增殖、成脂分化和胰岛素敏感性的影响。结果表明，SBO 增加细胞增殖能力，伴随着上调增殖细胞核抗原含量（p  < 0.05）和 p38 活性（p  < 0.05）。SBO 还促进脂肪生成并增强脂肪生成转录因子的表达。在 SBO 处理的细胞中，线粒体生物发生相关基因表达升高（p  < 0.05）。值得注意的是，SBO 还增加了葡萄糖摄取和葡萄糖转运蛋白 4 的丰度（p  < 0.05）。用 SBO 处理的细胞表现出更高的磷酸化胰岛素受体底物 1 ( p < 0.05)、磷酸化 Akt ( p  < 0.05) 和磷酸化 AMP 活化蛋白激酶 ( p  < 0.01) 含量。综合起来，这些结果表明 SBO 促进 3T3-L1 细胞增殖、脂肪生成和胰岛素敏感性。