In this study, we aimed to determine the potential functional properties and biological activities of the hydrolysates of perilla seed meal (PSM), which is a by-product of perilla seed oil extraction. PSM protein was hydrolyzed independently by using five proteases, and their functional and biological properties were analyzed. PSM protein hydrolysate exhibited high solubility at most of the tested pH values, and the trypsin-treated hydrolysate exhibited the highest water and oil absorption capacity. The neutrase-treated hydrolysate was most effective in scavenging the 1,1-diphenyl-2-picrylhydrazine radicals, whereas the pepsin-treated hydrolysate showed the highest angiotensin I-converting enzyme inhibitory effect, and anti-inflammatory activity. Trypsin-treated hydrolysate exhibited the highest scavenging activity against of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid radicals with the IC₅₀ of 109.72 µg/mL. The results of the present study suggest that the type of protease used for the treatment significantly influences the functional properties and biological activities of the resulting PSM protein hydrolysates.

在这项研究中，我们旨在确定紫苏籽粕 (PSM) 水解物的潜在功能特性和生物活性，它是紫苏籽油提取的副产品。使用五种蛋白酶独立水解PSM蛋白，并分析其功能和生物学特性。PSM 蛋白水解物在大多数测试的 pH 值下表现出高溶解度，胰蛋白酶处理的水解物表现出最高的吸水和吸油能力。中和酶处理的水解产物在清除 1,1-二苯基-2-苦基肼自由基方面最有效，而胃蛋白酶处理的水解产物显示出最高的血管紧张素 I 转换酶抑制作用和抗炎活性。胰蛋白酶处理的水解产物对 2 的清除活性最高，_{50 个}，共 109.72 µg/mL。本研究的结果表明，用于处理的蛋白酶类型显着影响所得 PSM 蛋白水解物的功能特性和生物活性。