The objectives of this study are to monitor the physicochemical properties of two freshwater lakes with different chemical characteristics and trophic status over a year (2019) and assess the bacterial diversity by a high-throughput sequencing method for a certain time. Carlson Trophic Index analysis revealed that, whereas the deep lake, Iznik Lake, (TSImean = 48.9) has mesotrophic characteristics, the shallow lake Manyas Lake (TSImean = 74.2) was found at a hypertrophic status. The most important parameters controlling water qualities in the lakes were temperature, alkalinity, and phosphate levels. Although the bacterial communities were dominated by the same phyla (Cyanobacteria, Bacteroidetes, Actinomicrobia, Proteobacteria, and Verrucomicrobia) in both lakes, the communities differed distinctly at the lower levels. Whereas Sporichthyaceae in Manyas Lake accounted for 10% of the total reads, the major share of the sequences was assigned to Cyanobacteria Family I (8%) in Iznik Lake. The hypertrophic Manyas Lake had a more diverse bacterial community rather than Iznik Lake and contained higher numbers of unique Operational Taxonomic Units.

中文翻译：

---

对比浅湖和深湖的水质和细菌群落模式：Manyas vs. Iznik

本研究的目的是在一年内（2019年）监测具有不同化学特征和营养状态的两个淡水湖泊的理化性质，并在一定时间内通过高通量测序方法评估细菌多样性。卡尔森营养指数分析显示，深湖伊兹尼克湖（TSImean = 48.9）具有中营养特征，而浅湖曼雅斯湖（TSImean = 74.2）则处于肥厚状态。控制湖泊水质的最重要参数是温度、碱度和磷酸盐水平。尽管两个湖泊中的细菌群落以相同的门（蓝藻门、拟杆菌门、放线菌门、变形菌门和疣微菌门）为主，但群落在较低水平上存在明显差异。马尼亚斯湖中的孢子科占总读数的 10%，而序列的主要份额被分配给伊兹尼克湖中的蓝藻家族 I (8%)。肥厚的马尼亚斯湖具有比伊兹尼克湖更多样化的细菌群落，并且包含更多数量的独特操作分类单元。