Biochar (char-product), generated by pyrolyzing organic materials, is produced for the intended use of land application to promote carbon sequestration, soil improvement and crop-yield. Despite the benefits biochar applications offers, scientific probing on impacts that may result from amendments with biochar is still fragmented. In this study, impact of biochar on Eudrilus eugeniae DNA was investigated. Rice-husk biochar was applied to soil at rates up to 80% d/w and earthworms were exposed for 35-day. Impact on DNA was measured using electrophoresis-gel-extraction-method. Data obtained showed that biochar application over 25% resulted in decreased survival. Electrophoresis-gel-analysis showed that DNA decreased from 450 to 300 bp in biochar soils (p = 0.002). Biochar rates (5%–25%) induced DNA damage. The DNA showed smeared bands or tail; indicating DNA degradation and/or damage. DNA damage is a clear evidence of negative impact of biochar(s) to soil-biota; suggesting that loading of soil with biochar could have serious consequences on soil-fauna.

通过热解有机材料而产生的生物炭（炭产物），专门用于土地用途，以促进碳固存，改善土壤和提高作物产量。尽管生物炭的应用提供了很多好处，但对生物炭修正可能产生的影响的科学探索仍然是零碎的。在这项研究中，研究了生物炭对Eudrilus eugeniae DNA的影响。稻壳生物炭以高达80％d / w的比例施用于土壤，and暴露35天。使用电泳-凝胶提取法测量对DNA的影响。获得的数据表明，生物炭施用量超过25％会导致存活率下降。电泳-凝胶分析表明，生物炭土壤中的DNA从450 bp降至300 bp（p = 0.002）。生物炭比率（5％–25％）引起DNA损伤。DNA显示出条带状或尾巴状。表明DNA降解和/或损坏。DNA损伤是生物炭对土壤生物群产生负面影响的明确证据。这表明用生物炭装载土壤可能会对土壤动物造成严重后果。