Five different inocula from cow manure, goat manure, pig manure, and rumen fluid (RU) and microbial sludge from anaerobic digestion were screened to select anaerobic lignocellulolytic microbial consortium (ALMC) of high efficacy in degradation of filter paper (FP), microcrystalline cellulose (MCC), and rice straw (RS) to produce methane. RU from Bubalus bubalis demonstrated the highest growth rate RU inoculum (10.45 mgVS/day); fastest degradation rate of FP, MCC, and RS (125.00, 50.07, and 25.43 mgVS/day); and highest methane production rate (6.23 ml/day). To obtain the enriched and stabilized ALMC from RU, consecutive batch subculturing was used and reached at 25 passages, namely, E25. The efficacies of E25 in RS removal and methane production were 78%VS and 293 ml/gVS_added compared with initial inoculum (E0) at 46% VS and 138 ml/gVS_added, respectively, within 14 days of incubation time. The efficacies of E25 in RS removal and methane production were 1.7- and 2.12-folds higher than of E0, respectively. E25 showed high ability to utilize various carbon sources (68%) from Biolog EcoPlate assay. It was confirmed that E25 could degrade derivatives of cellulose, hemicellulose, and lignin. Metagenomic analysis by 16 s and shotgun sequencing of E25 found dominant species of hydrolytic/fermentative bacteria (Proteiniphilum acetatigenes, Pyramidobacter piscolens, Syntrophus aciditrophicus, Mesotoga prima, and Candidatus Cloacimonas acidaminovorans) and methanogens (Methanoculleus bourgensis, Methanoculleus marisnigri, Methanofollis liminatans, Methanosarcina mazei, and Methanosaeta harundinacea). This stabilized E25 with enrichment of lignocellulolytic and fermentative bacteria as well as hydrogenotrophic and acetoclastic methanogens shows its efficacy in accelerating RS degradation and methane production.

筛选了来自牛粪，山羊粪，猪粪和瘤胃液（RU）的五种不同接种物，以及来自厌氧消化的微生物污泥，以选择对滤纸（FP），微晶纤维素降解具有高效功效的厌氧木质纤维素分解微生物联盟（ALMC）。 （MCC）和稻草（RS）产生甲烷。Bubalus bubalis的RU表现出最高的RU接种量增长率（10.45 mgVS / day）；FP，MCC和RS的最快降解速率（125.00、50.07和25.43 mgVS /天）；和最高的甲烷生产速度（6.23毫升/天）。为了从RU获得富集和稳定的ALMC，使用连续分批传代，并达到25代，即E25。E25在去除RS和产生甲烷方面的效率为78％VS，_添加了293 ml / gVS与孵育14天之内分别_添加46％VS和138 ml / gVS的初始接种物（E0）相比。E25在去除RS和甲烷生成方面的效率分别比E0高1.7倍和2.12倍。E25具有很高的利用Biolog EcoPlate分析中各种碳源的能力（68％）。证实了E25可以降解纤维素，半纤维素和木质素的衍生物。16个S和E25的鸟枪法测序的宏基因组分析，发现水解/发酵菌的优势种（Proteiniphilum acetatigenes，Pyramidobacter piscolens，酸养互营菌，Mesotoga初步和暂定Cloacimonas acidaminovorans）和产甲烷（Methanoculleus bourgensis，Methanoculleus marisnigri，Methanofollis liminatans，甲烷马氏产甲烷球菌，和Methanosaeta harundinacea）。这种稳定的E25具有丰富的木质纤维素分解和发酵细菌以及氢营养性和乙酰碎裂性产甲烷菌，显示了其加速RS降解和甲烷生成的功效。