Two-pore channels (TPCs) constitute a small family of cation channels that are localized in membranes of endosomal and lysosomal compartments. Although their roles for vesicular fusion and endolysosomal trafficking have been investigated, our knowledge on their expression pattern and higher order functions in the murine brain is still limited. Western blot analysis indicated a broad expression of TPC1 in the neocortex, cerebellum and hippocampus. In order to investigate the consequences of the genetic inactivation of TPC1, we performed a set of behavioural studies with TPC1^−/− mice. TPC1^−/− mice were analysed for an altered motor coordination and grip-strength, exploratory drive and anxiety as well as learning and memory. TPC1^−/− mice did not show any differences in their exploratory drive or in their anxiety levels. There were also no differences in spontaneous activity or motor performance. However, the Morris water maze test uncovered a deficit in spatial learning and memory in TPC1^−/− mice.

双孔通道 (TPC) 构成一个小家族的阳离子通道，位于内体和溶酶体隔室的膜中。尽管已经研究了它们在囊泡融合和内溶酶体运输中的作用，但我们对它们在小鼠大脑中的表达模式和更高阶功能的了解仍然有限。蛋白质印迹分析表明 TPC1 在新皮质、小脑和海马中广泛表达。为了研究 TPC1 基因失活的后果，我们对 TPC1 ^-/-小鼠进行了一组行为研究。分析了TPC1 ^-/-小鼠的运动协调和握力、探索性驱动和焦虑以及学习和记忆的改变。TPC1 ^-/-小鼠的探索动力或焦虑水平没有任何差异。自发活动或运动表现也没有差异。然而，Morris 水迷宫测试揭示了 TPC1 ^-/-小鼠空间学习和记忆的缺陷。