Surface plasmon resonance imaging (SPRI) detects the changes in refractive index in close proximity to the surface of a thin metal film as variations in light intensity reflected from the back of the film and thus does not require labeling for visualization of the structures under investigation. While traditionally, the wave vector scanning is performed via angular rotations, the wave vector can also be scanned though tuning of the wavelength. Here we demonstrate that a combination of a non-monochromatic electrically tunable bandpass filter in conjunction with highly chromatically corrected imaging objectives can yield subcellular resolution for imaging of the interior refractive index of human mesenchymal stem cells.

中文翻译：

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用于人骨髓细胞成像的表面等离子体共振

表面等离子共振成像 (SPRI) 检测靠近金属薄膜表面的折射率变化，作为从薄膜背面反射的光强度的变化，因此不需要标记来观察所研究的结构。传统上，波矢扫描是通过角旋转进行的，但也可以通过波长的调谐来扫描波矢。在这里，我们证明了非单色电可调带通滤波器与高度色彩校正的成像目标相结合，可以产生用于人类间充质干细胞内部折射率成像的亚细胞分辨率。