Background: As a substrate of apoER2, Reelin has been verified to exert neuroprotection by preventing memory impairment. Pinocembrin is the most abundant natural flavonoid found in propolis, and it has been used to exert neuroprotection, blood–brain barrier protection, anti-oxidation, and inflammation diminishing, both in vitro and in vivo. However, the roles and molecular mechanisms of pinocembrin in neurobehavioral outcomes and neuronal repair after vascular dementia are still under investigation.
Purpose: To explore the role of pinocembrin in the involvement of the Reelin-dab1 signaling pathway in improving memory impairment, both in cell culture and animals experiments.
Material and Methods: Behavioral tests were conducted on day 48 to confirm the protection of pinocembrin against cognitive impairment. Cell and molecular biology experiments demonstrated that the Reelin-dab1 pathway mediates the underlying mechanism of cognitive improvement by pinocembrin.
Results: It was showed that pinocembrin alleviated learning and memory deficits induced by vascular dementia, by inducing the expression of Reelin, apoER2, and p-dab1 in the hippocampus. The expression of Reelin and p-dab1 was both inhibited following Reelin RNA interference in SH-SY5Y prior to oxygen glucose deprivation (OGD) injury, suggesting that Reelin played a core role in pinocembrin’s effect on OGD in vitro.
Conclusion: Pinocembrin improves the cognition via the Reelin-dab1 signaling pathway.



中文翻译：

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Pinocembrin 改善由血管性痴呆引起的认知障碍：Reelin-dab1 信号通路的贡献。

背景：作为 apoER2 的底物，Reelin 已被证实通过防止记忆障碍发挥神经保护作用。Pinocembrin 是蜂胶中含量最丰富的天然类黄酮，在体外和体内均被用于发挥神经保护、血脑屏障保护、抗氧化和消炎作用。然而，松柏素在血管性痴呆后神经行为结果和神经元修复中的作用和分子机制仍在研究中。
目的：在细胞培养和动物实验中探索松松素在 Reelin-dab1 信号通路改善记忆障碍中的作用。
材料与方法：在第 48 天进行了行为测试，以确认松柏素对认知障碍的保护作用。细胞和分子生物学实验表明，Reelin-dab1 通路介导松松素改善认知的潜在机制。
结果：表明松松素通过诱导海马中Reelin、apoER2和p-dab1的表达来减轻血管性痴呆引起的学习和记忆障碍。Reelin 和 p-dab1 在氧葡萄糖剥夺 (OGD) 损伤前对 SH-SY5Y 进行 Reelin RNA 干扰后均受到抑制，表明 Reelin 在松松素体外对 OGD 的影响中起核心作用。
结论：松松素通过Reelin-dab1信号通路改善认知。