In this article, using the integration of paper microfluidics within laser-inscribed commercial contact lenses, we demonstrate the multiplexed detection of clinically relevant analytes including hydrogen ions, proteins, glucose, nitrites and L-ascorbic acid, all sampled directly from model tears. In vitro measurements involved the optimization of colorimetric assays, with readouts collected, stored and analyzed using a bespoke Tears Diagnostics smartphone application prototype. We demonstrate the potential of the device to perform discrete measurements either for medical diagnosis or disease screening in the clinic or at the point-of-care (PoC), with future applications including monitoring of ocular infections, uveitis, diabetes, keratopathies and assessing oxidative stress.

中文翻译：

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将纸质微流体传感器集成到隐形眼镜中进行泪液分析。

在本文中，利用纸微流体在激光刻印的商用隐形眼镜中的集成，我们证明了对与临床相关的分析物的多重检测，包括氢离子，蛋白质，葡萄糖，亚硝酸盐和L-抗坏血酸，所有这些都是直接从模型眼泪中取样的。体外测量涉及比色测定的优化，使用定制的Tears Diagnostics智能手机应用程序原型收集，存储和分析读数。我们展示了该设备在临床或现场护理（PoC）上进行医学诊断或疾病筛查的离散测量的潜力，其未来的应用包括监测眼部感染，葡萄膜炎，糖尿病，角膜病变和评估氧化性强调。