Mechanistic insights into toxicity pathways induced by nanomaterials in Daphnia magna from analysis of the composition of the acquired protein corona,Environmental Science: Nano

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Mechanistic insights into toxicity pathways induced by nanomaterials in Daphnia magna from analysis of the composition of the acquired protein corona
Environmental Science: Nano ( IF 5.8 ) Pub Date : 2020-09-04 , DOI: 10.1039/d0en00625d
Laura-Jayne A. Ellis _{1,

2,

3,

4} , Iseult Lynch _{1,

2,

3,

4}

Affiliation

Identifying proteins present on the surface of nanomaterials (NMs) incubated with plasma, serum, and cell lysates can offer insights into the biological interactions of the NMs, including uptake and toxicity. Organisms such as Daphnia magna respond to the presence of toxicants by secreting proteins and other biomolecules; we demonstrate that the eco-corona acquired during exposure to NMs can provide similar important insights regarding the mechanistic pathways induced by the NM exposure as part of an ecotoxicity assessment. Using freshly dispersed (pristine) and ‘medium aged’ NMs (as environmental pollutants), differing in core material, size and surface properties, the influence of NM physicochemical characteristics and exposure environment on the protein corona composition and potential for in vivo uptake by the model test species Daphnia magna are investigated. Surface bound protein corona compositions changed between NM type, NM ‘age’ and the environmental medium in which the NMs were incubated, thus changing the NM biological identity and interactions. Proteins identified on the freshly dispersed NM surfaces were largely associated with metabolic damage, DNA damage, mitochondrial breakdown and energy processes, all of which are associated with cytotoxic damage. Significantly fewer proteins were bound to the aged NMs in all medium conditions, compared to the freshly dispersed NMs. Proteins bound only to the aged NMs were involved in calcium ion binding and cell redox homeostasis, indicative of significantly lower toxic responses to the aged NMs. Thus, NM protein corona composition can facilitate detection of organism responses to NM exposure and potentially identification of the molecular initiating events in adverse outcome pathways.

中文翻译：

通过分析获得的蛋白质电晕的组成，了解水蚤中纳米材料诱导的毒性途径的机理。

鉴定与血浆，血清和细胞裂解液一起孵育的纳米材料（NMs）表面上存在的蛋白质，可以洞悉NMs的生物学相互作用，包括摄取和毒性。生物如水蚤到通过分泌的蛋白质和其它生物分子的存在毒物的响应; 我们证明，在暴露于NM期间获得的生态电晕可以提供类似的重要见解，作为由NM暴露作为生态毒性评估的一部分而引起的机械途径的重要见解。使用新鲜分散的（原始）和“中度老化”的NMs（作为环境污染物），它们的核心材料，大小和表面特性，NM理化特性和暴露环境对蛋白质电晕组成和潜在电势的影响不同模型测试种水蚤（Daphnia magna）在体内的吸收被调查。表面结合的蛋白质电晕组成在NM型，NM'年龄'和在其中孵育NM的环境介质之间改变，从而改变了NM的生物学特性和相互作用。在新近分散的NM表面鉴定出的蛋白质主要与代谢损伤，DNA损伤，线粒体分解和能量过程有关，所有这些都与细胞毒性损伤有关。与新鲜分散的NM相比，在所有中等条件下，与衰老的NM结合的蛋白质明显更少。仅与衰老的NMs结合的蛋白质参与钙离子结合和细胞氧化还原稳态，表明对衰老的NMs的毒性反应明显降低。从而，

更新日期：2020-11-03

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