Arboviruses cause the most devastating diseases in humans and animals worldwide. Several hundred arbovirus are transmitted by mosquitoes, sand flies or ticks and are responsible for more than million deaths annually. Development of a model system is essential to extrapolate the molecular events occurring during infection in the human and mosquito host. Virus overlay protein binding assay (VOPBA) combined with MALDI TOF/TOF MS revealed that Dengue-2 virus (DENV-2) exploits similar protein molecules in Drosophila melanogaster and Aedes aegypti for its infection. Furthermore, the virus susceptibility studies revealed that DENV-2 could propagate in D. melanogaster, and DENV-2 produced in fruit fly is equally infectious to D. melanogaster and Ae. aegypti. Additionally, real time PCR analysis revealed that RNAi coupled with JAK-STAT and Toll pathway constitutes an effector mechanism to control the DENV-2 infection in flies. These observations point out that D. melanogaster harbors all necessary machineries to support the growth of arboviruses. With the availability of well-established techniques for genetic and developmental manipulations, D. melanogaster, offers itself as the potential model system for the study of arbovirus-vector interactions.

中文翻译：

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果蝇（Drosophila melanogaster）作为研究虫媒病毒相互作用的模型宿主。

虫媒病毒在全世界的人类和动物中造成最严重的疾病。数百种虫媒病毒是通过蚊子，沙蝇或tick传播的，每年造成超过百万人死亡。建立模型系统对于推断人类和蚊子宿主在感染过程中发生的分子事件至关重要。病毒覆盖蛋白结合测定（VOPBA）与MALDI TOF / TOF MS结合显示，登革2病毒（DENV-2）利用果蝇和埃及伊蚊中相似的蛋白质分子进行感染。此外，病毒敏感性研究表明，DENV-2可以在D. melanogaster中繁殖，而在果蝇中产生的DENV-2对D. melanogaster和Ae具有相同的感染力。埃及。另外，实时PCR分析表明，RNAi结合JAK-STAT和Toll途径构成了控制蝇中DENV-2感染的效应器机制。这些发现指出，D。melanogaster拥有所有必要的机制来支持虫媒病毒的生长。凭借成熟的遗传和发育操作技术，黑腹果蝇（D. melanogaster）成为了研究虫媒病毒与载体相互作用的潜在模型系统。