The gut microbiota plays a crucial role in several physiologic functions of the host. In humans and animals, manipulation of the intestinal microbiota by oral administration of probiotic lactic acid bacteria plays a significant role in modulating the immune system. The aim of this study was to evaluate the safety of the probiotic mixture Slab51® and the capacity of this mixture to stimulate immune function in healthy dogs. Twenty dogs were divided in two groups and received a control diet or the same diet supplemented with a dose of 400 billion cfu of lyophilized bacteria for a period of 60 days. Body weight, food intake, body condition score (BCS), fecal score (FSS), fecal immunoglobulin IgA concentration, plasma IgG concentration, and fecal microbiota composition were monitored. Weight, food intake, BCS, FSS, and biochemical parameters remained unchanged during the treatment in both groups of animals. The fecal microbiota showed a significant decrease in the abundance of Clostridium perfringens and a significant increase in the abundance of beneficial Bifidobacterium and Lactobacillus organisms (p < 0.05). Fecal IgA and plasma IgG levels were significantly higher in the group receiving the probiotic compared to healthy controls. These data show that dietary supplementation with the probiotic mixture Slab51® is safe and well-tolerated, modulating the composition of the intestinal microbiota, and enhancing specific immune functions in healthy dogs.

肠道菌群在宿主的几种生理功能中起着至关重要的作用。在人类和动物中，通过口服益生菌乳酸菌来操纵肠道微生物群在调节免疫系统中起着重要作用。这项研究的目的是评估益生菌混合物Slab51®的安全性以及该混合物刺激健康狗的免疫功能的能力。将二十只狗分为两组，并接受对照饮食或相同饮食，并补充4000亿cfu冻干细菌剂量，持续60天。监测体重，食物摄入，身体状况评分（BCS），粪便评分（FSS），粪便免疫球蛋白IgA浓度，血浆IgG浓度和粪便微生物群组成。体重，食物摄入量，BCS，FSS，在治疗过程中，两组动物的生化参数均保持不变。粪便菌群的丰度显着降低。产气荚膜梭菌 并且有益双歧杆菌和乳杆菌生物的丰度大大增加（p<0.05）。与健康对照组相比，接受益生菌的组的粪便中IgA和血浆IgG水平明显更高。这些数据表明，益生菌混合物Slab51®的膳食补充是安全且耐受良好的，可调节肠道菌群的组成，并增强健康犬的特定免疫功能。