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Use of Drones to Recover Fungal Spores and Pollen from the Lower Atmosphere
Caribbean Journal of Science ( IF 0.4 ) Pub Date : 2020-06-01 , DOI: 10.18475/cjos.v50i1.a16
Zuleimary Vélez-Rodríguez 1 , Hernán Torres-Pratts 2 , Sandra L. Maldonado-Ramírez 3
Affiliation  

Aerobiological studies focus on studying biological particulates such as pollen and fungal spores in the lower atmosphere. Dispersion of these bio particulates is widespread, and their capture can be difficult, requiring the development and use of different methods to achieve their capture. For this research, a modified unmanned aerial vehicle, commonly known as drone, fitted with remote-controlled spore traps was used to collect spores and pollen from the lower atmosphere. Samplings of five minutes of flight with the samplers open and Petri dishes exposed to collect viable particulate were made above two areas with different anthropogenic activity. Adhesive slides were exposed simultaneously to collect non-viable particulate. From viable samples, common fungi such as Cladosporium, Curvularia, Fusarium, Penicillium, Aspergillus, and Nigrospora were identified. From non-viable samples, 54 genera of fungal spores and 17 pollen genera were observed directly under the microscope. Spores of Delitschia, Solheimia, Venturia, Fusariella, Endophragmiella, and Polythrincium are reported here as new records for Puerto Rico.

中文翻译:

使用无人机从低层大气中回收真菌孢子和花粉

航空生物学研究的重点是在低层大气中研究诸如花粉和真菌孢子等生物微粒。这些生物微粒的分散是广泛的,并且它们的捕获可能是困难的,需要开发和使用不同的方法来实现它们的捕获。为了进行这项研究,使用了一种装有遥控孢子捕集器的改进型无人机(通常称为无人机),用于从低层大气中收集孢子和花粉。在两个具有不同人为活动的区域上方,打开采样器并暴露于皮氏培养皿以收集可行的颗粒,进行飞行五分钟的采样。同时暴露出粘性载玻片,以收集不可行的颗粒。从可行的样品中,常见的真菌,如枝孢菌,弯孢菌,镰刀菌,青霉菌,曲霉菌,和Nigrospora被确定。从不可行的样品中,直接在显微镜下观察到54个真菌孢子和17个花粉属。据报道,熟食店,Solheimia,Venturia,Fusariella,Endophragmiella和Polythrincium的孢子是波多黎各的新记录。
更新日期:2020-06-01
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