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N, S, and P-Co-doped Carbon Quantum Dots: Intrinsic Peroxidase Activity in a Wide pH Range and Its Antibacterial Applications
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-09-03 , DOI: 10.1021/acsbiomaterials.0c00831 Kumud Malika Tripathi 1 , Hee Tae Ahn 2 , Minsoo Chung 2 , Xuan Ai Le 2 , Deepika Saini 3 , Anshu Bhati 3 , Sumit Kumar Sonkar 3 , Moon Il Kim 2 , TaeYoung Kim 4
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-09-03 , DOI: 10.1021/acsbiomaterials.0c00831 Kumud Malika Tripathi 1 , Hee Tae Ahn 2 , Minsoo Chung 2 , Xuan Ai Le 2 , Deepika Saini 3 , Anshu Bhati 3 , Sumit Kumar Sonkar 3 , Moon Il Kim 2 , TaeYoung Kim 4
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Nanozymes have drawn significant scientific interest due to their high practical importance in terms of overcoming the instability, complicated synthesis, and high cost of protein enzymes. However, their activity is generally limited to particular pHs, especially acidic ones. Herein, we report that luminescent N, S, and P-co-doped carbon quantum dots (NSP-CQDs) act as attractive peroxidase mimetics in a wide pH range, even at neutral pH, for the peroxidase substrate 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in the presence of H2O2. The synergistic effects of multiple heteroatoms doping in CQDs boost the catalytic activity in a wide pH range attributed to the presence of high density of active sites for enzymatic-like catalysis and accelerated electron transfer during the peroxidase-like reactions. A possible reaction mechanism for the peroxidase-like activity of CQDs is investigated based on the radical trapping experiments. Moreover, the multifunctional activity of NSP-CQDs was further utilized for antibacterial assays for both Gram-negative and Gram-positive model species, including Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), respectively. The growths of the employed E. coli and S. aureus were found to be significantly inhibited due to the peroxidase-mediated perturbation of cell walls. The present work signifies the current advance in the rational design of N, S, and P-co-doped CQDs as highly active peroxidase mimics for novel applications in diverse fields, including catalysis, medical diagnostics, environmental chemistry, and biotechnology.
中文翻译：
N，S和P-Co掺杂的碳量子点：很宽的pH范围内在过氧化物酶活性及其抗菌应用
纳米酶由于其在克服蛋白质酶的不稳定性，复杂的合成和高成本方面的高度实用性而引起了广泛的科学兴趣。然而，它们的活性通常限于特定的pH，尤其是酸性pH。本文中，我们报道了发光的N，S和P共掺杂的碳量子点（NSP-CQDs）在过大的pH范围内，甚至在中性pH值下，都可作为有吸引力的过氧化物酶模拟物，用于过氧化物酶底物2,2'-azino H 2 O 2存在下的-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）。CQDs中多个杂原子掺杂的协同效应提高了在宽pH范围内的催化活性，这归因于存在高密度的酶促催化活性位点和过氧化物酶样反应过程中加速了电子转移。基于自由基捕获实验，研究了CQDs过氧化物酶样活性的可能反应机理。此外，NSP-CQDs的多功能活性被进一步用于革兰氏阴性和革兰氏阳性模型物种的抗菌测定，分别包括大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）。受雇的大肠杆菌和金黄色葡萄球菌的生长发现由于过氧化物酶介导的细胞壁扰动而被显着抑制。目前的工作标志着N，S和P共掺杂的CQD作为高活性过氧化物酶模拟物的合理设计的最新进展，可用于多种领域的新应用，包括催化，医学诊断，环境化学和生物技术。
更新日期：2020-10-12
中文翻译：
N，S和P-Co掺杂的碳量子点：很宽的pH范围内在过氧化物酶活性及其抗菌应用
纳米酶由于其在克服蛋白质酶的不稳定性，复杂的合成和高成本方面的高度实用性而引起了广泛的科学兴趣。然而，它们的活性通常限于特定的pH，尤其是酸性pH。本文中，我们报道了发光的N，S和P共掺杂的碳量子点（NSP-CQDs）在过大的pH范围内，甚至在中性pH值下，都可作为有吸引力的过氧化物酶模拟物，用于过氧化物酶底物2,2'-azino H 2 O 2存在下的-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）。CQDs中多个杂原子掺杂的协同效应提高了在宽pH范围内的催化活性，这归因于存在高密度的酶促催化活性位点和过氧化物酶样反应过程中加速了电子转移。基于自由基捕获实验，研究了CQDs过氧化物酶样活性的可能反应机理。此外，NSP-CQDs的多功能活性被进一步用于革兰氏阴性和革兰氏阳性模型物种的抗菌测定，分别包括大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）。受雇的大肠杆菌和金黄色葡萄球菌的生长发现由于过氧化物酶介导的细胞壁扰动而被显着抑制。目前的工作标志着N，S和P共掺杂的CQD作为高活性过氧化物酶模拟物的合理设计的最新进展，可用于多种领域的新应用，包括催化，医学诊断，环境化学和生物技术。
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