Weaning is known to be the most stressful and critical transition experienced by lambs, it affects the structure of the gastrointestinal tract microbiomes, which significantly influences the lamb’s health and performance. The present study identified the structure of lambs' pre- and post-weaning fecal microbiomes to define the implications of the weaning transition. Illumina MiSeq yielded a total of 5169 sequence reads per fecal sample ranging from 106 to 2028. Before weaning, the taxonomic analysis indicated that the phylum Firmicutes dominated other bacterial phyla at a rate of (≤52.47%), followed by Proteobacteria at a rate of (≤52.00%). On the contrary, the phylum Firmicutes (≤93.01%) tended to increase, while the phylum Proteobacteria tended to decrease (≤26.83 %) after weaning. At the genus level, twenty-eight genera assigned to three phyla were detected in pre-weaned lambs; meanwhile, thirty-three genera assigned to four phyla were identified in post-weaned lambs. These findings suggested that weaning significantly influenced the diversity and the abundance of lambs' fecal microbiota.

断奶是羔羊经历的最紧张和最关键的过渡期，它影响胃肠道微生物群的结构，从而显着影响羔羊的健康和性能。本研究确定了羔羊断奶前和断奶后粪便微生物组的结构，以定义断奶过渡的含义。Illumina MiSeq在106至2028年的每个粪便样品中共获得5169个序列读数。断奶前，分类学分析表明，Firmicutes门以其他细菌门占主导（≤52.47％），其次是Proteobacteria。 （≤52.00％）。相反，断奶后门扇菌门（≤93.01％）趋于增加，而Proteobacteria门菌门趋于减少（≤26.83％）。在属上 在断奶的羔羊中检测到28个属，分为3个门。同时，在断奶后的羔羊中鉴定出33个属，分为4个门。这些发现表明，断奶显着影响了羔羊粪便微生物群的多样性和丰度。