Abstract

Context

Metabolic syndrome (MetS) is a clustering of several physiological alterations.

Objective

This study was designed to evaluate the effects of MetS on rats spermatogenesis and steroidogenesis.

Materials and methods

We developed a MetS rodent model using high-sugar and high-fat diet.

Results

MetS rats showed severe disorders in sperm parameters. Interestingly, a significant increase in malondialdehyde level and a decrease in the antioxidant activities were observed. Moreover, qRT-PCR analysis showed Bax down-regulation and Bcl-2 up-regulation. A decrease in testosterone level was identified, correlated with the CYP11A1, CYP17A1 and 17β HSD testicular marker down-regulation. Finally, MetS rats showed an up-regulation of pro-inflammatory cytokines receptors IL-1R and IL-6R.

Conclusion

MetS induced severe testis toxicity in male rats. Mets markedly distorted sperm parameters, inhibited the transcription of steroidogenic enzymes and led to oxidative stress, inflammation, and alteration of Bax/Bcl-2 ratioin testicular tissues.

中文翻译：

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代谢综合征大鼠模型中的精子发生和类固醇生成中断

摘要

语境

代谢综合征 (MetS) 是几种生理改变的集合。

客观的

本研究旨在评估 MetS 对大鼠精子发生和类固醇生成的影响。

材料和方法

我们开发了一种使用高糖和高脂肪饮食的 MetS 啮齿动物模型。

结果

MetS 大鼠的精子参数出现严重紊乱。有趣的是，观察到丙二醛水平显着增加和抗氧化活性降低。此外，qRT-PCR 分析显示 Bax 下调和 Bcl-2 上调。鉴定了睾酮水平的降低，其与CYP11A1、CYP17A1和17β HSD睾丸标志物下调相关。最后，MetS 大鼠表现出促炎细胞因子受体IL-1R和IL-6R的上调。

结论

MetS 在雄性大鼠中引起严重的睾丸毒性。大都会显着扭曲精子参数，抑制类固醇生成酶的转录，并导致睾丸组织中的氧化应激、炎症和 Bax/Bcl-2 比率的改变。