Fruit set is the process whereby ovaries develop into fruits after pollination and fertilization. The process is induced by the phytohormone gibberellin (GA) in tomatoes, as determined by the constitutive GA response mutant procera. However, the role of GA on the metabolic behavior in fruit-setting ovaries remains largely unknown. This study explored the biochemical mechanisms of fruit set using a network analysis of integrated transcriptome, proteome, metabolome, and enzyme activity data. Our results revealed that fruit set involves the activation of central carbon metabolism, with increased hexoses, hexose phosphates, and downstream metabolites, including intermediates and derivatives of glycolysis, the tricarboxylic acid cycle, and associated organic and amino acids. The network analysis also identified the transcriptional hub gene SlHB15A, that coordinated metabolic activation. Furthermore, a kinetic model of sucrose metabolism predicted that the sucrose cycle had high activity levels in unpollinated ovaries, whereas it was shut down when sugars rapidly accumulated in vacuoles in fruit-setting ovaries, in a time-dependent manner via tonoplastic sugar carriers. Moreover, fruit set at least partly required the activity of fructokinase, which may pull fructose out of the vacuole, and this could feed the downstream pathways. Collectively, our results indicate that GA cascades enhance sink capacities, by up-regulating central metabolic enzyme capacities at both transcriptional and posttranscriptional levels. This leads to increased sucrose uptake and carbon fluxes for the production of the constituents of biomass and energy that are essential for rapid ovary growth during the initiation of fruit set.

坐果是卵巢在授粉和受精后发育成果实的过程。该过程由番茄中的植物激素赤霉素（GA）诱导，这是由组成型GA反应突变体procera确定的。然而，GA在坐果卵巢中对代谢行为的作用仍然未知。这项研究通过综合的转录组，蛋白质组，代谢组和酶活性数据的网络分析，探索了水果的生化机制。我们的研究结果表明，坐果涉及到中央碳代谢的激活，增加了己糖，己糖磷酸盐和下游代谢产物的含量，包括糖酵解的中间体和衍生物，三羧酸循环以及相关的有机和氨基酸。网络分析还确定了转录中心基因SlHB15A，即协调新陈代谢的活化。此外，蔗糖代谢的动力学模型预测蔗糖循环在未授粉的卵巢中具有高活性水平，而当糖通过结瘤糖载体以时间依赖的方式快速累积在结实的卵巢中的液泡中时，其被关闭。此外，坐果至少部分地需要果糖激酶的活性，其可能将果糖从液泡中拉出，这可以为下游途径提供营养。总体而言，我们的结果表明，GA级联通过在转录和转录后水平上调中央代谢酶的能力来增强库的能力。