A microwave‐assisted extraction (MAE) method was developed for the extraction of bioactive inositols (D‐chiro‐ and myo‐inositols) from lettuce (Lactuca sativa) leaves as a strategy for the revalorization of these agrofood residues. Gas chromatography‐mass spectrometry was selected for the simultaneous determination of inositols and sugars (glucose, fructose, and sucrose) in these samples. A Box–Behnken experimental design was used to maximize the extraction of inositols based on the results of single factor tests. Optimal conditions of the extraction process were as follows: liquid‐to‐solid ratio of 100:1 v/w, 40°C, 30 min extraction time, 20:80 ethanol:water (v/v), and one extraction cycle. When compared with conventional solid‐liquid extraction (SLE), MAE was found to be more effective for the extraction of target bioactive carbohydrates (MAE 5.42 mg/g dry sample versus SLE 4.01 mg/g dry sample). Then, MAE methodology was applied to the extraction of inositols from L. sativa leaves of different varieties (var. longifolia, var. capitata and var. crispa). D‐chiro‐ and myo‐inositol contents varied between 0.57–7.15 and 0.83–3.48 mg/g dry sample, respectively. Interfering sugars were removed from the extracts using a biotechnological procedure based on the use of Saccharomyces cerevisiae for 24 h. The developed methodology was a good alternative to classical procedures to obtain extracts enriched in inositols from lettuce residues, which could be of interest for the agrofood industry.

中文翻译：

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开发微波辅助提取方法，用于从生菜（Lactuca sativa）副产品中回收生物活性肌醇。


开发了一种微波辅助提取 (MAE) 方法，用于从生菜 ( Lactuca sativa ) 叶子中提取生物活性肌醇（D- chiro 肌醇和myo肌醇），作为这些农产品残留物重新评估的策略。选择气相色谱-质谱法同时测定这些样品中的肌醇和糖（葡萄糖、果糖和蔗糖）。根据单因素测试的结果，采用 Box-Behnken 实验设计来最大限度地提取肌醇。最佳提取工艺条件为：液固比100:1 v/w、40°C、提取时间30 min、乙醇:水(v/v) 20:80、提取1个周期。与传统的固液萃取 (SLE) 相比，MAE 对目标生物活性碳水化合物的提取更有效（MAE 5.42 mg/g 干样品与 SLE 4.01 mg/g 干样品）。然后，应用MAE方法从不同品种的苜蓿（var. longifolia 、var. capitata和var. Crispa ）叶中提取肌醇。 D-手性肌醇和肌醇含量分别在 0.57-7.15 和 0.83-3.48 mg/g 干样品之间变化。使用基于酿酒酵母24 小时的生物技术程序从提取物中去除干扰糖。所开发的方法是从生菜残渣中获取富含肌醇的提取物的经典程序的良好替代方案，这可能会引起农产品行业的兴趣。