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Saccharolysis of laccase delignified Aloe vera leaf rind and fermentation through free and immobilized yeast for ethanol production
Journal of Food Process Engineering ( IF 2.7 ) Pub Date : 2020-09-04 , DOI: 10.1111/jfpe.13514 Gunasekaran Rajeswari 1 , Samuel Jacob 1
Journal of Food Process Engineering ( IF 2.7 ) Pub Date : 2020-09-04 , DOI: 10.1111/jfpe.13514 Gunasekaran Rajeswari 1 , Samuel Jacob 1
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Aloe vera gel has found prominent application in food, therapeutic and cosmetics manufacturing that eventually increased the generation of leaf rind as processing waste. This biomass was previously delignified by laccase to have better accessibility of holocellulose and an attempt has been made through this study to optimize enzymatic hydrolysis using crude cellulase (5.25 [U/ml]) produced from Aspergillus sp. A maximum saccharification of 63.00 ± 0.45% was achieved under the optimum conditions (solid‐to‐liquid ratio 1:17 [wt/vol], 53°C and 8.5 hr) with sugar yield of 309 ± 2.1 mg/g. Fermentation of saccharified liquid to ethanol using Saccharomyces cerevisiae (yeast) was performed using three different modes of inoculum application such as free cell suspension; Ca‐alginate immobilized yeast suspension and immobilized yeast loaded in a packed bed reactor (PBR) under uniform process conditions. Immobilized yeast in PBR has resulted in maximum ethanol yield of about 16.50 ± 1.02 g/L with a fermentation efficiency of 76.14%.
中文翻译：
漆酶脱木素的芦荟叶皮的糖解和通过游离的固定化酵母发酵生产乙醇
芦荟凝胶已在食品，治疗和化妆品生产中得到了广泛应用，最终增加了作为加工废料的叶皮的产生。该生物质先前被漆酶脱木质素以具有更好的全纤维素可及性，并且已经通过该研究尝试使用由曲霉属（Aspergillus sp。）产生的粗纤维素酶（5.25 [U / ml]）来优化酶促水解。在最佳条件下（固液比1:17 [wt / vol]，53°C和8.5 hr），最大糖化度为63.00±0.45％，糖产量为309±2.1 mg / g。使用酿酒酵母将糖化液发酵为乙醇（酵母）使用三种不同的接种方式进行接种，例如游离细胞悬浮液；海藻酸钙固定化酵母悬浮液和固定化酵母在均匀工艺条件下装载在填充床反应器（PBR）中。固定在PBR中的酵母产生的最大乙醇产量约为16.50±1.02 g / L，发酵效率为76.14％。
更新日期：2020-09-04
中文翻译：
漆酶脱木素的芦荟叶皮的糖解和通过游离的固定化酵母发酵生产乙醇
芦荟凝胶已在食品，治疗和化妆品生产中得到了广泛应用，最终增加了作为加工废料的叶皮的产生。该生物质先前被漆酶脱木质素以具有更好的全纤维素可及性，并且已经通过该研究尝试使用由曲霉属（Aspergillus sp。）产生的粗纤维素酶（5.25 [U / ml]）来优化酶促水解。在最佳条件下（固液比1:17 [wt / vol]，53°C和8.5 hr），最大糖化度为63.00±0.45％，糖产量为309±2.1 mg / g。使用酿酒酵母将糖化液发酵为乙醇（酵母）使用三种不同的接种方式进行接种，例如游离细胞悬浮液；海藻酸钙固定化酵母悬浮液和固定化酵母在均匀工艺条件下装载在填充床反应器（PBR）中。固定在PBR中的酵母产生的最大乙醇产量约为16.50±1.02 g / L，发酵效率为76.14％。
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