This study evaluated the potential of a microplasma UV lamp as an alternative UV source to the current mercury-based (Hg-based) UV lamp for water disinfection. We developed a set of PCR-based molecular assays (long-range qPCR, DNase, and binding assay) to quantify the adenovirus genome, capsid, and fiber damage with a wide detection range (10^0.5-10^6.5 PFU/mL). We used these molecular assays to characterize adenovirus (AdV) inactivation kinetics by microplasma UV that produced monochromatic UV at 222 nm. We found that the inactivation rate constant (0.142 cm²/mJ) due to microplasma UV was 4.4 times higher than that of low-pressure Hg UV (0.032 cm²/mJ). This high efficacy was attributed to monochromatic UV wavelength at 222 nm damaging the AdV capsid protein. The results of these molecular assays also proved that microplasma UV and medium-pressure Hg UV with a bandpass filter at 223 nm (MPUV_223nm) have a similar influence on AdV (p>0.05). We then estimated the relative energy efficiency of MPUV and microplasma UV to LPUV for 4 log reduction of the viruses. We found that the microplasma UV resulted in higher inactivation rate constants for viruses than the current Hg-based UV. Consequently, microplasma UV could be more energy efficient than low-pressure Hg UV for water disinfection if the wall-plug efficiency of the microplasma UV lamp improved to 8.4% (currently 1.5%). Therefore, the microplasma UV lamp is a promising option for water disinfection.

这项研究评估了微等离子体紫外线灯作为目前水消毒汞基（Hg基）紫外线灯替代紫外线源的潜力。我们开发了一套基于PCR的分子测定法（远程qPCR，DNase和结合测定法），以较宽的检测范围（10 ^0.5 -10 ^6.5 PFU / mL）定量检测腺病毒基因组，衣壳和纤维损伤。我们使用这些分子测定法来表征由微等离子体UV产生的222 nm单色UV的腺病毒（AdV）灭活动力学。我们发现，由于等离子紫外线导致的失活速率常数（0.142 cm ² / mJ）比低压汞紫外线（0.032 cm ^2）高4.4倍。/ mJ）。这种高功效归因于在222 nm处的单色UV波长损坏了AdV衣壳蛋白。这些分子测定的结果还证明，在223 nm（MPUV 223 nm ）带通滤光片的微_等离子体UV和中压Hg UV对AdV具有相似的影响（p> 0.05）。然后，我们估计了MPUV和微等离子体UV对LPUV的相对能量效率，使病毒减少4 log。我们发现，与当前基于汞的紫外线相比，微等离子体紫外线导致更高的病毒灭活速率常数。因此，如果将微等离子体紫外线灯的壁塞效率提高到8.4％（目前为1.5％），则微等离子体紫外线比用于水消毒的低压Hg紫外线更具能源效率。因此，微等离子体紫外线灯是用于水消毒的有前途的选择。