Abstract Horticultural products, including peaches, are susceptible to chilling injury (CI), on which the effect of ethylene is still controversial, and the underlying mechanism remains elusive. Here, changes in biochemical and molecular mechanisms involved in phenolic and lipid metabolism were compared between stony hard peaches with continuous ethylene (CETH) and controls. CETH effectively compromised internal browning incidence, accompanied by the inhibited activity of peroxidase but enhanced phenolic content, and less membrane leakage with reduced H2O2 and malondialdehyde contents. Intriguingly, CETH elevated levels of phospholipids and unsaturation of their acyl chains, coincident with the lower transcript levels of phospholipase Dα1 but higher fatty acid desaturase2/8.1, and the enhanced sphingolipid contents and biosynthesis concomitant with higher transcript levels of glucosylceramide synthase but lower inositol phosphorylceramide synthase3. Therefore, CETH can ameliorate fruit CI through adjusting phenolic and lipid metabolism, especially comprehensive remodeling of phospholipids and sphingolipids to contribute to the membrane stability.

中文翻译：

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连续乙烯调节磷脂和鞘脂有助于石硬桃果实的耐寒性

摘要 包括桃子在内的园艺产品易受冷害（CI）的影响，乙烯对冷害的影响尚存在争议，其潜在机制尚不清楚。在这里，比较了含有连续乙烯 (CETH) 的石质硬桃和对照之间参与酚类和脂质代谢的生化和分子机制的变化。CETH 有效地降低了内部褐变的发生率，伴随着过氧化物酶的活性受到抑制，但酚类含量增加，膜渗漏减少，H2O2 和丙二醛含量降低。有趣的是，CETH 提高了磷脂水平和其酰基链的不饱和度，与较低的磷脂酶 Dα1 转录水平和较高的脂肪酸去饱和酶 2/8.1 的转录水平一致，鞘脂含量和生物合成的增加伴随着葡萄糖神经酰胺合成酶的转录水平较高，但肌醇磷酸神经酰胺合成酶的转录水平较低3。因此，CETH可以通过调节酚类和脂类代谢，尤其是磷脂和鞘脂的综合重构来改善果实的CI，从而有助于膜的稳定性。