Abstract Nuclear factor (NF)-Y is composed of three subunits, NF-YA, NF-YB and NF-YC, and plays important roles in the regulation of plant abiotic stress response gene expression. However, the function of individual soybean NF-YA proteins in response to drought stress is unclear. In this study, the soybean NF-YA subunit gene GmNF-YA10 was cloned, and its function in response to drought stress was identified. GmNF-YA10 is broadly expressed in soybean tissues and is specifically located in the nucleus. In soybean roots, the transcription level of the GmNF-YA10 gene was induced by polyethylene glycol (PEG)-simulated drought stress and abscisic acid (ABA) treatment. Under mannitol stress, the germination rate of transgenic Arabidopsis overexpressing GmNF-YA10 was higher than that of wild-type (WT) Arabidopsis. After drought stress, the survival rate, relative water content, ABA content, proline content and chlorophyll content of transgenic Arabidopsis overexpressing GmNF-YA10 were higher than those of WT Arabidopsis while the rate of water loss and the contents of malondialdehyde and sucrose were lower than those of WT Arabidopsis, indicating that transgenic Arabidopsis overexpressing GmNF-YA10 has better drought resistance than WT Arabidopsis. Under the ABA treatment, the root length of transgenic Arabidopsis overexpressing GmNF-YA10 was shorter than that of WT Arabidopsis and the degree of stomatal closure was greater than that of WT Arabidopsis, indicating that transgenic Arabidopsis overexpressing GmNF-YA10 was more sensitive to ABA at the post-germination stage. Transcriptome sequencing and real-time quantitative PCR analysis showed that GmNF-YA10 affected the expression of both ABA-dependent and ABA-independent signalling pathway genes. These results indicate that GmNF-YA10 significantly improves the drought resistance of transgenic Arabidopsis through ABA-dependent and ABA-independent signal transduction pathways.

中文翻译：

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大豆核因子 YA10 正调控转基因拟南芥的抗旱性

摘要 核因子(NF)-Y由NF-YA、NF-YB和NF-YC三个亚基组成，在植物非生物胁迫响应基因表达的调控中发挥重要作用。然而，个别大豆 NF-YA 蛋白在应对干旱胁迫时的功能尚不清楚。本研究克隆了大豆 NF-YA 亚基基因 GmNF-YA10，并鉴定了其响应干旱胁迫的功能。GmNF-YA10 在大豆组织中广泛表达，并且特异地位于细胞核中。在大豆根中，GmNF-YA10 基因的转录水平由聚乙二醇 (PEG) 模拟的干旱胁迫和脱落酸 (ABA) 处理诱导。在甘露醇胁迫下，过表达 GmNF-YA10 的转基因拟南芥的萌发率高于野生型（WT）拟南芥。干旱胁迫后，成活率、过表达 GmNF-YA10 的转基因拟南芥的相对含水量、ABA 含量、脯氨酸含量和叶绿素含量均高于 WT 拟南芥，而失水率、丙二醛和蔗糖含量均低于 WT 拟南芥，表明转基因过表达 GmNF-YA10 的拟南芥比 WT 拟南芥具有更好的抗旱性。在ABA处理下，过表达GmNF-YA10的转基因拟南芥根长短于WT拟南芥，气孔闭合程度大于WT拟南芥，说明过表达GmNF-YA10的转基因拟南芥对ABA更敏感。发芽后阶段。转录组测序和实时定量 PCR 分析表明 GmNF-YA10 影响 ABA 依赖性和非 ABA 依赖性信号通路基因的表达。这些结果表明 GmNF-YA10 通过 ABA 依赖性和非 ABA 依赖性信号转导途径显着提高了转基因拟南芥的抗旱性。