Xanthan is one of the most important biopolymers characterised by a high commercial value. Since the availability of essential nutrients influences its biosynthesis, the aim of this study was the optimisation of carbon, organic and inorganic nitrogen content in glycerol-based medium employing response surface methodology. The cultivation of strain Xanthomonas campestris ATCC 13951 was carried out under appropriate conditions on media with glycerol, peptone and ammonium-nitrate formulated according to Box-Behnken design (33), while the desirability function approach was used for determination of optimal nutrient levels. The final model predicts that the maximal amount of xanthan (12.95 g/L) is produced when the initial contents of glycerol, peptone and ammonium-nitrate in the medium are 32.96 g/L, 0.55 g/L and 0.73 g/L, respectively. To minimize the residual nutrient content and therefore the costs of effluent processing, additional optimisation was performed. In order to validate the optimisation model developed and examine the bioprocess success with crude glycerol as the sole carbon source, additional experiments were performed. The results represent reliable information for further investigations.

中文翻译：

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使用响应面方法优化基于甘油的培养基上的黄原胶生产

黄原胶是最重要的生物聚合物之一，具有很高的商业价值。由于必需营养素的可用性影响其生物合成，因此本研究的目的是使用响应面方法优化基于甘油的培养基中的碳、有机和无机氮含量。菌株 Xanthomonas campestris ATCC 13951 的培养是在适当条件下在根据 Box-Behnken 设计 (33) 配制的甘油、蛋白胨和硝酸铵的培养基上进行的，同时使用合意性函数方法来确定最佳营养水平。最终模型预测，当培养基中甘油、蛋白胨和硝酸铵的初始含量分别为 32.96 g/L、0.55 g/L 和 0.73 g/L 时，产生最大量的黄原胶 (12.95 g/L) . 为了最大限度地减少残留养分含量，从而减少污水处理的成本，进行了额外的优化。为了验证开发的优化模型并检查以粗甘油作为唯一碳源的生物过程的成功，进行了额外的实验。结果代表了进一步调查的可靠信息。