Key message

Expression analysis of Larix kaempferi mature miR171s and their primary transcripts and target gene LaSCL6 during somatic embryogenesis revealed the transcriptional and post-transcriptional regulation of the miR171-LaSCL6 module.

Abstract

Somatic embryogenesis provides a useful experimental system for studying the regulatory mechanisms of plant development. The level and activity of microRNA171 (miR171) fluctuate during somatic embryogenesis in Larix kaempferi, but the underlying mechanisms are still unclear. Here, in L. kaempferi we identified five members of the miR171 family, which cleave LaSCL6 mRNA at different sites. In addition, we improved the method of measuring miRNA activity in a more direct way. Furthermore, we measured the expression patterns of mature miR171s and their primary transcripts during somatic embryogenesis in L. kaempferi and found that their patterns differed, indicating that the transcription of MIR171 genes and the subsequent cleavage of their intermediate products are regulated. Taken together, our findings not only offer a means to study the regulation of miRNA activity, but also provide further insight into the regulation of L. kaempferi somatic embryogenesis by miR171-LaSCL6.

中文翻译：

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落叶松体细胞胚发生过程中miR171-LaSCL6模块的转录和转录后调控

关键信息

落叶松成熟miR171s及其初级转录本和靶基因LaSCL6在体细胞胚发生过程中的表达分析揭示了miR171- LaSCL6模块的转录和转录后调控。

抽象

体细胞胚发生为研究植物发育的调控机制提供了有用的实验系统。在落叶松体细胞胚发生过程中，microRNA171（miR171）的水平和活性会发生变化，但其潜在机制仍不清楚。在这里，我们在kaempferi中鉴定了miR171家族的五个成员，它们在不同位点切割LaSCL6 mRNA。另外，我们改进了以更直接的方式测量miRNA活性的方法。此外，我们测量了成熟的miR171s及其初级转录本在L. kaempferi体细胞胚发生过程中的表达模式，发现它们的模式不同，表明MIR171的转录基因和其中间产物的后续切割受到调控。综上所述，我们的发现不仅提供了研究miRNA活性调控的方法，而且还提供了对L调控的进一步认识。miR171- LaSCL6产生的kaempferi体细胞胚发生。