Systemic treatments for ischemic stroke as a disease with high disability and death have been yet unsuccessful. Combined treatments can potentially cause better results in treatment of patients with Stroke. In this study we assessed the neuroprotective effect of modafinil-coated gold nanoparticles (AuNPs) and mesenchymal stem cell (MSC) in ischemic stroke rats. Stem cells and AuNPs offer great promise for new medical treatments. 60 male Wistar rats were randomly divided into five groups (12 in each group): (1) the group that developed middle cerebral artery occlusion (MCAO or ischemia), (2) the normal group (control), (3) the MCAO group that received MSC (C + MCAO), (4) the MCAO group that received MSC and modafinil (CM + MCAO), and (5) the MCAO group that received MSC and modafinil-coated AuNPs (CMN + MCAO). Middle Cerebral Artery Occlusion (MCAO) was performed by inserting a silicone coat filament in the right internal carotid artery via the external carotid artery until it reached the anterior cerebral artery. The filament was located in the internal carotid artery for 60 min and then removed. Modafinil-coated AuNPs (100 mg/kg) or Modafinil (100 mg/kg) were given to the rats as an oral gavage, once a day in the morning time. Finally, infarct volume, BDNF (Brain-derived neurotrophic factor), GDNF (Glial cell-derived neurotrophic factor), NeuN (neuronal nuclear protein) expression, and cell apoptosis in brain were analyzed. The brain infarct volume and apoptosis significantly decreased and BDNF, NeuN, and GDNF increased in C + MCAO, CM + MCAO, and CMN + MCAO groups compared to ischemia. CMN + MCAO groups did not show significant difference in these factors compared to control group. These results demonstrated that the administration of stem cells and Modafinil-coated AuNPs at the same time had a good effect on ischemic brain injuries. It happened through increasing neurotrophic factors and decreasing brain cell apoptosis.

缺血性中风作为一种高度残疾和死亡的疾病的系统治疗尚未成功。综合治疗可能会在中风患者的治疗中带来更好的效果。在这项研究中，我们评估了莫达非尼涂层金纳米颗粒（AuNPs）和间充质干细胞（MSC）在缺血性中风大鼠中的神经保护作用。干细胞和AuNPs为新的医学治疗提供了广阔的前景。将60只雄性Wistar大鼠随机分为五组（每组12只）：（ 1）发生大脑中动脉闭塞（MCAO或局部缺血）的组，（2）正常组（对照组），（3）MCAO组（4）接受MSC和莫达非尼（CM + MCAO）的MCAO组，和（5）接受MSC和莫达非尼包衣的AuNP（CMN + MCAO）的MCAO组。大脑中动脉闭塞（MCAO）是通过将硅橡胶涂层细丝经颈外动脉插入右颈内动脉直至到达大脑前动脉来进行的。将细丝置于颈内动脉中60分钟，然后取出。将莫达非尼包衣的AuNPs（100 mg / kg）或莫达非尼（100 mg / kg）口服灌胃，每天早上一次。最后，分析了梗死体积，BDNF（脑源性神经营养因子），GDNF（胶质细胞源性神经营养因子），NeuN（神经核蛋白）的表达以及脑细胞凋亡。脑梗死体积和细胞凋亡明显减少，将莫达非尼包衣的AuNPs（100 mg / kg）或莫达非尼（100 mg / kg）口服灌胃，每天早上一次。最后，分析了梗死体积，BDNF（脑源性神经营养因子），GDNF（胶质细胞源性神经营养因子），NeuN（神经核蛋白）的表达以及脑细胞凋亡。脑梗死体积和细胞凋亡明显减少，将莫达非尼包衣的AuNPs（100 mg / kg）或莫达非尼（100 mg / kg）口服灌胃，每天早晨一次。最后，分析了梗死体积，BDNF（脑源性神经营养因子），GDNF（胶质细胞源性神经营养因子），NeuN（神经核蛋白）的表达以及脑细胞凋亡。脑梗死体积和细胞凋亡明显减少，与缺血相比，C + MCAO，CM + MCAO和CMN + MCAO组的BDNF，NeuN和GDNF升高。与对照组相比，CMN + MCAO组在这些因素上没有显示出显着差异。这些结果表明，同时施用干细胞和莫达非尼包被的AuNPs对缺血性脑损伤具有良好的效果。它通过增加神经营养因子和减少脑细胞凋亡而发生。