The efficacy of Allium sativum and Cannabis sativa against Rhipicephalus microplus ticks was evaluated using the adult immersion and the larval packet test. In addition, an in silico approach was utilized by performing a docking study in order to identify the active ingredients from both plants. Results showed a comparatively high lethal effect of A. sativum and C. sativa on egg laying (index of egg laying = 0.26 and 0.24, respectively), egg hatching (33.5 and 37.1, respectively), and total larval mortality (100%, both), at 40 mg/mL. When applied to cattle which had been inoculated with larvae ticks, it was observed that a 45% solution of both herbal extracts significantly reduced the number of ticks by 96 h post treatment. We analyzed in silico 27 known active molecules from both plants and identified in the PubChem database to explore the hypothesis that the effect found on ticks was based on inhibition of acetylcholinesterase (AChE). Vitamin E and cannabidiol are the most potent AChE inhibitors with docking scores of -15.85 and -14.38, respectively. Based on these findings, we conclude that A. sativum and C. sativa may potentially be used for the control of R. microplus, and should be further investigated as a potential supplement to or replacement of synthetic acaricides.

的功效大蒜和大麻对扇头蜱microplus使用成人浸没和幼虫分组试验蜱进行评价。另外，通过进行对接研究来利用计算机方法，以鉴定来自两种植物的活性成分。结果表明A具有较高的致死作用。大蒜和Ç。苜蓿产蛋量（产蛋指数分别为0.26和0.24），孵化蛋（分别为33.5和37.1）和总幼虫死亡率（均为100％），均为40 mg / mL。当将其应用于已接种幼虫tick的牛时，观察到两种草药提取物的45％溶液在处理后96 h均可显着减少tick的数量。我们在计算机上分析了来自这两种植物的27种已知活性分子，并在PubChem数据库中进行了鉴定，以探索关于tick的作用基于抑制乙酰胆碱酯酶（AChE）的假设。维生素E和大麻二酚是最有效的AChE抑制剂，对接分数分别为-15.85和-14.38。基于这些发现，我们得出结论：A。sativum和C. sativa可能被用于控制R. microplus，并应作进一步研究，以作为合成杀螨剂的潜在补充或替代。