Abstract

The present report deals with the identification of new bacteria for the degradation of sulphonated azo dye such as methyl orange because this type of dye is difficult to degrade by the physico-chemical method. Hence, an eco-friendly and efficient technique like biological method was studied to degrade methyl orange. Initially, three strains were isolated from the flasks containing media amended with textile wastewater using the serial dilution process. Out of three strains, micrococcus yunnanensis exhibited a higher decolourization efficacy in 100 mg L⁻¹, and hence, this strain was chosen for further optimization studies and metabolites identification. This is the first report to study the micrococcus species in methyl orange degradation according to our knowledge. The optimum parameters for the decolourization of methyl orange were found to be pH 7, temperature at 30 °C and dye concentration at 100 mg L⁻¹. The carbon source and nitrogen source suited well for the decolourization were observed as 1% glucose and 1% peptone. The metabolites obtained after degradation of methyl orange were analysed using FT-IR and GC–MS analysis. The final product obtained from the degraded sample was found to be N, N-Diethyl-N’-methyl-1,3-propane diamine.

Graphic abstract

中文翻译：

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使用新型细菌菌株及其代谢物表征对水溶液中甲基橙染料脱色的可持续方法

摘要

本报告涉及用于降解诸如甲基橙之类的磺化偶氮染料的新细菌的鉴定，因为这种染料很难通过物理化学方法降解。因此，研究了诸如生物方法的生态友好且有效的技术以降解甲基橙。最初，使用连续稀释工艺从装有培养基的烧瓶中分离出三个菌株，该培养基用纺织废水进行了修正。在三种菌株中，云南微球菌在100 mg L ^-1中表现出更高的脱色功效，因此，该菌株被选作进一步的优化研究和代谢物鉴定。这是根据我们的知识研究甲基橙降解中的微球菌物种的第一份报告。发现用于甲基橙脱色的最佳参数是pH 7，温度在30°C和染料浓度在100 mg L ^-1。观察到非常适合脱色的碳源和氮源为1％的葡萄糖和1％的蛋白ept。使用FT-IR和GC-MS分析了甲基橙降解后获得的代谢产物。发现从降解的样品获得的最终产物是N，N-二乙基-N′-甲基-1，3-丙烷二胺。

图形摘要