Microbial processes are being developed to transform flavonoid glycosides to varieties of metabolites with higher bioavailability. The aim of this study was to determine the metabolic activity and survival of five lactic acid bacteria (LAB) stains (L. rhamnosus LRa05, L. casei LC89, L. plantarum N13, L. acidophilus LA85, and L. brevis LB01) in two different citrus flavanone standards (hesperetin-7-O-rutinoside and naringenin-7-O-rutinoside). The enzymatic activity, metabolites, antioxidant activities, and α-glucosidase inhibition property in the two standards were also investigated before and after incubated with LAB. The medium contained standards permitted survival of the five LAB stains. All strains exhibited β-glucosidase activity. Of the five LAB strains tested, just L. plantarum N13 and L. brevis LB01 have the ability to metabolize hesperetin-7-O-rutinoside, only L. plantarum N13, L. acidophilus LA85, and L. brevis LB01 could metabolize naringenin-7-O-rutinoside, moreover, L. acidophilus LA85l was the strain with the highest biotransformation ratio of naringenin-7-O-rutinoside. L. acidophilus LA85 and L. plantarum N13 can degrade naringenin-7-O-rutinoside into naringenin. L. brevis LB01 can degrade hesperetin-7-O-rutinoside into hesperetin, 3-(4′-hydroxyphenyl)-2-propenoic acid, 3-(3′-hydroxy-4′-methoxyphenyl)hydracrylic acid, and 3-(4′-hydroxyphenyl)propionic acid. Incubation of L. acidophilus LA85 in naringenin-7-O-rutinoside solution supposed no apparent influence in the biological activities that tested. L. acidophilus LA85 may potentially contribute to the bioavailability of citrus flavanones, and to be applied as functional cultures to obtain more bioavailable and bioactive metabolites in food products or in the human gastrointestinal tract.

正在开发微生物过程以将类黄酮糖苷转化为具有更高生物利用度的各种代谢物。本研究的目的是确定五种乳酸菌 (LAB) 染色剂（鼠李糖乳杆菌LRa05、干酪乳杆菌LC89、植物乳杆菌N13、嗜酸乳杆菌LA85 和短乳杆菌）的代谢活性和存活率LB01) 在两种不同的柑橘黄烷酮标准品（橙皮素-7-O-芸香苷和柚皮素-7-O-芸香苷）中。还研究了两种标准品在与 LAB 孵育前后的酶活性、代谢物、抗氧化活性和 α-葡萄糖苷酶抑制特性。培养基中含有允许五种 LAB 染色剂存活的标准品。所有菌株均表现出β-葡萄糖苷酶活性。在测试的五种 LAB 菌株中，只有植物乳杆菌N13 和短乳杆菌LB01 具有代谢橙皮素-7-O-芸香苷的能力，只有植物乳杆菌N13、嗜酸乳杆菌LA85 和短乳杆菌LB01 能够代谢柚皮素- 7-O-芸香苷，此外，嗜酸乳杆菌LA85l是柚皮素-7-O-芸香苷生物转化率最高的菌株。L. acidophilus LA85 和L. plantarum N13 可以将 naringenin-7-O- rutinoside降解为柚皮素。L. brevis LB01 可将橙皮素-7-O-芸香苷降解为橙皮素、3-(4'-羟基苯基)-2-丙烯酸、3-(3'-羟基-4'-甲氧基苯基)羟基丙烯酸和3-( 4'-羟基苯基）丙酸。L. acidophilus LA85 在 naringenin-7-O-rutinoside 溶液中的培养假定对所测试的生物活性没有明显影响。嗜酸乳杆菌 LA85 可能有助于提高柑橘黄烷酮的生物利用度，并可用作功能性培养物，以在食品或人体胃肠道中获得更多的生物利用度和生物活性代谢物。