Background/Aim: Hepatoma-derived growth factor (HDGF) is involved in the progression of hepatocellular carcinoma (HCC). The present study assessed the epigenomic changes in hepatoma-derived cells through HDGF stimulation. Materials and Methods: We used two hepatoma-derived cell lines (HepG2 and SK-Hep1) and searched for microRNAs whose expression commonly changed in response to HDGF administration. We further explored a genetic database to investigate the association of the candidate microRNAs with the survival of HCC patients. Results: Despite both HepG2 and SK-Hep1 cells being categorized as hepatoma-derived cells, the microRNA profile differed between these two lines. However, HepG2 and SK-Hep1 cells shared 30 up-regulated and 2 down-regulated microRNAs. Of these, miR-6072 and miR-3137 were significantly associated with a poor prognosis in HCC patients. Conclusion: We identified two candidate microRNAs whose expression increased in response to HDGF stimulation. Both these molecules were associated with a poor prognosis of HCC patients.

中文翻译：

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肝细胞源性生长因子刺激诱导的肝细胞癌相关 microRNAs

背景/目的：肝癌衍生生长因子 (HDGF) 参与肝细胞癌 (HCC) 的进展。本研究通过 HDGF 刺激评估了肝癌细胞的表观基因组变化。材料和方法：我们使用了两种源自肝癌的细胞系（HepG2 和 SK-Hep1），并寻找其表达通常随着 HDGF 给药而改变的 microRNA。我们进一步探索了一个遗传数据库，以研究候选 microRNA 与 HCC 患者生存的关联。结果：尽管 HepG2 和 SK-Hep1 细胞都被归类为肝癌细胞，但这两个细胞系的 microRNA 谱不同。然而，HepG2 和 SK-Hep1 细胞共享 30 个上调和 2 个下调的 microRNA。这些，miR-6072 和 miR-3137 与 HCC 患者的不良预后显着相关。结论：我们鉴定了两种候选 microRNA，它们的表达响应 HDGF 刺激而增加。这两种分子都与 HCC 患者的不良预后有关。