Background/Aim: The transient receptor potential vanilloid 1 (TRPV1) ion receptor is involved in the release of calcitonin gene-related peptide (CGRP), a major contributor to orthodontic pain. Approaches that attenuate expression of TRPV1 and CGRP may reduce orthodontic pain. We explored the ability of high-frequency interval vibration to reduce orthodontic pain. Materials and Methods: Orthodontic force (50 g) was applied to both maxillary first molars in 8-week-old Wistar rats (n=72). Vibration was applied at 125 Hz for 15 min/day. Duration of face grooming was assessed as a measure of orthodontic pain. Immunofluorescence and western blotting were used to assess TRPV1 and CGRP in the trigeminal ganglia. Results: Compared to orthodontic force alone, application of vibration significantly decreased the duration of face grooming at 24 h and day 3 and reduced expression of TRPV1 and CGRP at 24 h. Conclusion: Vibration represents a promising mechanical approach to reduce orthodontic pain.

中文翻译：

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间隔振动通过涉及下调 TRPV1 和 CGRP 的机制减轻正畸疼痛

背景/目的：瞬时受体电位香草素 1 (TRPV1) 离子受体参与降钙素基因相关肽 (CGRP) 的释放，这是导致正畸疼痛的主要因素。减弱 TRPV1 和 CGRP 表达的方法可以减轻正畸疼痛。我们探索了高频间歇振动减轻正畸疼痛的能力。材料和方法：对 8 周大的 Wistar 大鼠 (n=72) 的两个上颌第一磨牙施加正畸力 (50 g)。以 125 Hz 的频率振动 15 分钟/天。面部修饰的持续时间被评估为正畸疼痛的量度。免疫荧光和蛋白质印迹用于评估三叉神经节中的 TRPV1 和 CGRP。结果：与单纯的正畸力相比，振动的应用显着减少了 24 小时和第 3 天的面部修饰持续时间，并降低了 24 小时 TRP​​V1 和 CGRP 的表达。结论：振动是一种有前景的减少正畸疼痛的机械方法。