Background/Aim: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined whether expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular anti-oxidative capacity. Materials and Methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation. Results: Heat and H2O2 stimulation resulted in ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX. Conclusion: Introduction of cAPX increases the anti-oxidative capacity in mammalian cells.

中文翻译：

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Cyanidioschyzon merolae 来源的抗坏血酸过氧化物酶在哺乳动物细胞中的表达

背景/目的：来自 Cyanidioschyzon merolae 的抗坏血酸过氧化物酶 (APX)，一种生活在高温和酸性环境中的原始红藻，比其他植物中存在的类似过氧化物酶具有更强的抗氧化能力。在本研究中，我们检查了花青素衍生的 APX (cAPX) 在哺乳动物细胞中的表达是否会增加细胞的抗氧化能力。材料与方法：将cAPX基因导入小鼠成纤维细胞样细胞系C3H10T1/2。在热、H2O2 和酸刺激后评估活性氧 (ROS) 的产生和/或细胞活力。结果：热和 H2O2 刺激导致 ROS 产生。与缺乏 cAPX 的对照细胞相比，表达 cAPX 的细胞更能耐受由热、H2O2 和酸刺激诱导的氧化应激。结论：