We studied the effect of optical clearing (OC) by glycerol, Omnipaque TM (iohexol), and polyethylene glycol on human skin in vivo using a portable Raman spectroscopy system. Kinetic curves were determined for different concentrations of these optical clearing agents (OCAs). The three agents enhanced OC, showing non-monotonic behavior for serial concentrations. The dehydration mechanism also caused a decay in OC during the initial 10−20 min. Furthermore, characteristic Raman signals could be used to identify different areas of skin and tissues. Variations in different skin sites predominantly affected the Raman bands at 462, 683, 883, 1253, 1415, 1450, and 1655 cm −1 .

中文翻译：

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便携式拉曼光谱法在体内研究人类皮肤的光学清洁动力学

我们使用便携式拉曼光谱系统研究了甘油，Omnipaque TM（iohexol）和聚乙二醇对人体皮肤的光学清除（OC）的影响。测定这些光学清除剂（OCA）的不同浓度的动力学曲线。这三种试剂增强了OC，显示了连续浓度下的非单调行为。在最初的10-20分钟内，脱水机制还导致OC的衰减。此外，特有的拉曼信号可用于识别皮肤和组织的不同区域。不同皮肤部位的变化主要影响拉曼光谱带在462、683、883、1253、1415、1450和1655 cm -1处。