To fully elucidate the roles and mechanisms of plant hormones in leaf senescence, we adopted an integrated analysis of both non-senescing and senescing leaves from red maple with transcriptome and metabolome data. Transcription and metabolite profiles were generated through a combination of deep sequencing, third-generation sequencing data analysis, and ultrahigh-performance liquid chromatograph Q extractive mass spectrometry (UHPLC-QE-MS), respectively. We investigated the accumulation of compounds and the expression of biosynthesis and signaling genes for eight hormones. The results revealed that ethylene and abscisic acid concentrations increased during the leaf senescence process, while the contents of cytokinin, auxin, jasmonic acid, and salicylic acid continued to decrease. Correlation tests between the hormone content and transcriptional changes were analyzed, and in six pathways, genes closely linked with leaf senescence were identified. These results will enrich our understanding of the mechanisms of plant hormones that regulate leaf senescence in red maple, while establishing a foundation for the genetic modification of Acer in the future.

中文翻译：

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综合转录组学和代谢组学分析红槭发育叶片衰老过程中激素途径。


为了充分阐明植物激素在叶片衰老中的作用和机制，我们对红枫的非衰老和衰老叶片进行了转录组和代谢组数据的综合分析。分别通过深度测序、第三代测序数据分析和超高效液相色谱 Q 提取质谱 (UHPLC-QE-MS) 的组合生成转录和代谢物谱。我们研究了八种激素的化合物积累以及生物合成和信号基因的表达。结果表明，叶片衰老过程中乙烯和脱落酸浓度增加，而细胞分裂素、生长素、茉莉酸和水杨酸含量持续下降。通过分析激素含量与转录变化之间的相关性测试，在6个途径中鉴定出与叶片衰老密切相关的基因。这些结果将丰富我们对调节红枫叶片衰老的植物激素机制的理解，同时为未来宏碁的基因改造奠定基础。