Background. Neuroinflammation plays a key role in myocardial ischemia-reperfusion (I/R) injury. Previous studies showed that light-emitting diode (LED) therapy might improve M2 microglia activation and brain-derived neurotrophic factor (BDNF) expression, thereby exerting anti-inflammatory effects. Therefore, we hypothesized that LED therapy might reduce myocardial I/R injury by neuroinflammation modulation. Objective. To explore the effect of LED therapy on myocardial I/R-induced injury and seek the underlying mechanism. Methods. Thirty rats were randomly divided into three groups: Control group (without LED treatment or myocardial I/R, ), I/R group (with myocardial I/R only, ), and LED+I/R group (with myocardial I/R and LED therapy, ). Electrocardiogram was recorded continuously during the procedure. In addition, brain tissue was extracted for BDNF, Iba1, and CD206 analyses, and heart tissue for myocardial injury (ischemic size and infarct size), IL-4 and IL-10 mRNA analysis. Results. In comparison with the I/R group, the ischemia size and the infarct size were significantly attenuated by LED therapy in the LED+I/R group. Meanwhile, the microglia activation induced by I/R injury was prominently attenuated by LED treatment either. And it is apparent that there was also an increase in the beneficial neuroinflammation markers (BDNF and CD206) in the paraventricular nucleus (PVN) in the LED+I/R group. Furthermore, the anti-inflammatory cytokines, IL-4 and IL-10, were greatly decreased by I/R while improved by LED treatment in myocardium. Conclusion. LED therapy might reduce neuroinflammation in PVN and decrease myocardium injury by elevating BDNF and M2 microglia.

中文翻译：

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发光二极管疗法通过减轻神经炎症来防止心肌缺血/再灌注损伤。

背景。神经炎症在心肌缺血再灌注 (I/R) 损伤中起关键作用。先前的研究表明，发光二极管 (LED) 疗法可能会改善 M2 小胶质细胞的活化和脑源性神经营养因子 (BDNF) 的表达，从而发挥抗炎作用。因此，我们假设 LED 疗法可能通过调节神经炎症来减少心肌 I/R 损伤。客观。探讨 LED 治疗对心肌 I/R 损伤的影响并寻找其潜在机制。方法。30 只大鼠随机分为三组：对照组（未进行 LED 治疗或心肌 I/R，)， I/R 组（仅心肌 I/R，）和 LED+I/R 组（心肌 I/R 和 LED 治疗，）。手术过程中连续记录心电图。此外，提取脑组织用于 BDNF、Iba1 和 CD206 分析，并提取心脏组织用于心肌损伤（缺血大小和梗塞大小）、IL-4 和 IL-10 mRNA 分析。结果. 与 I/R 组相比，LED+I/R 组通过 LED 治疗显着减轻缺血面积和梗死面积。同时，由 I/R 损伤引起的小胶质细胞活化也被 LED 治疗显着减弱。很明显，LED+I/R 组的室旁核 (PVN) 中的有益神经炎症标志物（BDNF 和 CD206）也有所增加。此外，抗炎细胞因子 IL-4 和 IL-10 通过 I/R 显着降低，而 LED 治疗改善心肌。结论。LED 疗法可能通过提升 BDNF 和 M2 小胶质细胞来减少 PVN 中的神经炎症并减少心肌损伤。