A microfluidic chip with a microvalve based on a microhole array is proposed in this paper for the POCT of tumor marker proteins. In order to control the biochemical reaction time accurately and obtain a higher testing sensitivity, the parameters of the microhole array are optimized basing on the investigation of the effects of the variation of those parameters on the fluid rate and the residual liquid value in the microvalve region. By conducting liquid flow experiments using microvalves based on microhole arrays with varying microstructural parameters, the residual rate of reaction products is demonstrated to be proportional to the depth and diameter of the microholes and inversely proportional to the distance between the microhole centers. A comprehensive analysis indicates that a microhole depth of 95 μm, a microhole diameter of 230 μm, and a distance between microhole centers of 250 μm not only ensure a sufficiently long delay time, but also reduce the residual rate of reaction products, thereby providing an optimum microvalve performance that maximizes the detection efficiency and accuracy of microfluidic chips.

中文翻译：

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基于微孔芯片的微阀性能优化。

本文提出了一种基于微孔阵列的微阀微流控芯片，用于肿瘤标记蛋白的POCT。为了精确地控制生化反应时间并获得更高的测试灵敏度，在研究微孔阵列的参数变化对微阀区域的流体速率和剩余液体值的影响的基础上，对微孔阵列的参数进行了优化。 。通过使用基于具有不同微结构参数的微孔阵列的微阀进行液体流动实验，证明了反应产物的残留率与微孔的深度和直径成正比，与微孔中心之间的距离成反比。综合分析表明，微孔深度为 95μ米，230一个微孔直径 μ m和250微孔中心之间的距离 μ中号不仅确保足够长的延迟时间，同时也降低反应产物的残留率，由此提供最大化的检测效率的最佳微阀性能和微流控芯片的准确性。