Local Environment Affects the Activity of Enzymes on a 3D Molecular Scaffold.,ACS Nano

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Local Environment Affects the Activity of Enzymes on a 3D Molecular Scaffold.
ACS Nano ( IF 15.8 ) Pub Date : 2020-09-03 , DOI: 10.1021/acsnano.0c03962
Yan Xiong ₁ , James Huang ₂ , Shih-Ting Wang ₃ , Sufi Zafar ₄ , Oleg Gang _{1,

2,

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Affiliation

The ability to coordinate and confine enzymes presents an opportunity to affect their performance and to create chemically active materials. Recent studies show that polymers and biopolymers can be used to scaffold enzymes, and that can lead to the modulated biocatalytic efficiency. Here, we investigated the role of microenvironments on enzyme activity using a well-defined molecular scaffold. An enzyme, glucose oxidase (GOx), was positioned at different locations of a three-dimensional (3D) octahedral DNA scaffold (OS), allowing the enzyme’s polyanionic environments to be altered. Using electrical sensing, based on a bipolar junction transistor, we measured directly and in real-time the enzyme’s proton generation at these different microenvironments. We found a 200% enhancement of immobilized enzyme over free GOx and about a 30% increase in catalytic rates when the enzyme was moved on the same molecular scaffold to a microenvironment with a higher local concentration of polyanions, which suggests a role of local pH on the enzymatic activity.

中文翻译：

本地环境影响3D分子支架上酶的活性。

协调和限制酶的能力为影响酶的性能和产生化学活性物质提供了机会。最近的研究表明，聚合物和生物聚合物可用于支架酶，并且可以导致调节的生物催化效率。在这里，我们使用定义明确的分子支架研究了微环境对酶活性的作用。一种酶，葡萄糖氧化酶（GOx），位于三维（3D）八面体DNA支架（OS）的不同位置，可以改变该酶的聚阴离子环境。使用基于双极结型晶体管的电感应，我们直接并实时测量了在这些不同微环境下酶的质子生成。

更新日期：2020-09-03

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