Organophosphates are a large class of chemicals with anticholinesterase action insecticides. Dimethoate belongs to the class of organophosphates and it is used for agriculture purpose. Its main toxicological role in animals and humans is the inhibition of the activity of acetylcholinesterase. Although it is not considered genotoxic, carcinogenic and teratogen, there is evidence of increased pup mortality in developmental neurotoxicity studies. Since there is scant published literature about developmental toxicity, we investigated the adverse effects of dimethoate on fertilization and embryonic development in sea urchin (Paracentrotus lividus), a model organism widely used to assess the toxicity of contaminants on environmental matrices; so pesticide residues can be released into the environment, and could affect the health of organisms, including humans. Different solution of dimethoate (4 × 10⁻³, 4 × 10⁻⁴, 4 × 10⁻⁵, 4 × 10⁻⁶ and 4 × 10⁻⁷ g/10 ml) have been tested on spermatozoa of P. lividus to evaluate the fertilizing ability of them when we added egg cells untreated. We demonstrated that dimethoate does not interfere with fertilizing ability of spermatozoa but egg cells fertilized by treated spermatozoa showed alterations in the segmentation planes as asymmetric and/or asynchronous cell divisions.

中文翻译：

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乐果毒对受精和 Paracentrotus lividus 胚胎发育的评估 (Lamarck, 1816)。

有机磷是一大类具有抗胆碱酯酶作用的杀虫剂。乐果属于有机磷酸盐类，用于农业目的。它在动物和人类中的主要毒理学作用是抑制乙酰胆碱酯酶的活性。虽然它不被认为具有遗传毒性、致癌性和致畸性，但在发育神经毒性研究中有证据表明幼崽死亡率增加。由于关于发育毒性的已发表文献很少，我们研究了乐果对海胆（Paracentrotus lividus）受精和胚胎发育的不利影响。)，一种广泛用于评估污染物对环境基质毒性的模式生物；因此，农药残留会释放到环境中，并可能影响包括人类在内的生物体的健康。不同的乐果溶液（4 × 10 ^-3、4 × 10 ^-4、4 × 10 ^-5、4 × 10 ^-6和 4 × 10 ^-7  g/10 ml）已在青色假单胞菌精子上进行测试以评估当我们添加未经处理的卵细胞时，它们的受精能力。我们证明乐果不会干扰精子的受精能力，但由处理过的精子受精的卵细胞显示出分割平面的改变，如不对称和/或异步细胞分裂。