2Naphthalene sulfonate (2NS) is an intermediate compound used in textile industries. Being nonbiodegradable, the concerns regarding its biotoxicity have risen. In the present investigation the toxic effects of 2NS were analyzed with the help of Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR), which was used to monitor changes in the vibrational modes of functional groups within the biomolecules. After calculating LD _50, one half of LD ₅₀ i.e. 0.33 mg/15 g b.w. was intraperitoneally administrated and the brain tissue was collected for investigation after 96 h of exposure. The spectra observed revealed the significant differences in absorbance and areas between control and treated groups reflecting the change in proteins, lipids and nucleic acid due to toxicity induced by 2NS. In addition, protein secondary structure analysis was focused in this study, which reveals alterations in α helix and β sheet structure after 2NS intoxication. Histopathology of brain was also studied, which reveals changes in the histology of brain in group treated with 2NS. In conclusion, the study highlighted the application of ATR-FTIR and histopathology for toxicity assessment.

中文翻译：

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使用 ATR-FTIR 改变生物分子结构和暴露于 2Naphthalene sufonate 的 Channa punctatus 脑组织的组织病理学变化。

2萘磺酸盐 (2NS) 是一种用于纺织工业的中间化合物。由于不可生物降解，对其生物毒性的担忧有所增加。在本研究中，借助衰减全反射傅里叶变换红外 (ATR-FTIR) 来分析 2NS 的毒性作用，该方法用于监测生物分子内官能团振动模式的变化。计算LD后_50， LD的一半₅₀即，腹腔注射 0.33 毫克/15 克体重，并在暴露 96 小时后收集脑组织用于研究。观察到的光谱揭示了对照组和处理组之间吸光度和面积的显着差异，反映了由于 2NS 诱导的毒性引起的蛋白质、脂质和核酸的变化。此外，本研究重点分析了蛋白质二级结构，揭示了 2NS 中毒后 α 螺旋和 β 折叠结构的改变。还研究了大脑的组织病理学，这揭示了 2NS 治疗组大脑组织学的变化。总之，该研究强调了 ATR-FTIR 和组织病理学在毒性评估中的应用。