当前位置: X-MOL 学术Toxicol. Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A moderate physiological dose of benzyl butyl phthalate exacerbates the high fat diet-induced diabesity in male mice.
Toxicology Research ( IF 2.2 ) Pub Date : 2020-06-12 , DOI: 10.1093/toxres/tfaa037
Jian Zhang 1 , Catherine A Powell 1 , Matthew K Kay 1 , Min Hi Park 1 , Sunitha Meruvu 1 , Ravi Sonkar 1 , Mahua Choudhury 1
Affiliation  

Exposure to endocrine disrupting chemicals (EDCs) used in plastic manufacturing processes may be contributing to the current increase in metabolic disorders. Here, we determined that benzyl butyl phthalate (BBP), a common EDC and food packaging plasticizer, mixed into chow diet (CD) and high fat diets (HFD) at varying concentrations (4 μg/kg body weight (bw)/day, 169 μg/kg bw/day, 3 mg/kg bw/day, 50 mg/kg bw/day) produced a number of detrimental and sex-specific metabolic effects in C57BL/6 male and female mice after 16 weeks. Male mice exposed to moderate (3 mg/kg bw/day) concentrations of BBP in an HFD were especially affected, with significant increases in body weight due to significant increases in weight of liver and adipose tissue. Other doses did not show any significant changes when compared to only CD or HFD alone. HFD in the presence of 3 mg/kg bw/day BBP showed significant increases in fasting blood glucose, glucose intolerance, and insulin intolerance when compared to HFD alone. Furthermore, this group significantly alters transcriptional regulators involved in hepatic lipid synthesis and its downstream pathway. Interestingly, most of the BBP doses had no phenotypic effect when mixed with CD and compared to CD alone. The female mice did not show a similar response as the male population even though they consumed a similar amount of food. Overall, these data establish a dose which can be used for a BBP-induced metabolic research model and suggest that a moderate dosage level of EDC exposure can contribute to widely ranging metabolic effects.

中文翻译:

中度生理剂量的邻苯二甲酸苄基丁酯会加剧高脂饮食诱导的雄性小鼠的肥胖。

暴露于塑料制造过程中使用的内分泌干扰化学物质(EDC)可能会导致当前代谢紊乱的增加。在这里,我们确定了邻苯二甲酸苄基丁酯(BBP)(一种常见的EDC和食品包装增塑剂)以不同的浓度(4μg/ kg体重(bw)/天)混入食物(CD)和高脂饮食(HFD)中, 169μg/ kg体重/天,3 mg / kg体重/天,50 mg / kg体重/天)在16周后对C57BL / 6雄性和雌性小鼠产生了许多有害和性别特异性的代谢作用。暴露于HFD中度(3 mg / kg bw /天)中度BBP的雄性小鼠尤其受到影响,由于肝脏和脂肪组织重量的显着增加,体重显着增加。与仅使用CD或HFD相比,其他剂量未显示任何显着变化。与单独的HFD相比,存在3 mg / kg bw /天BBP的HFD显示空腹血糖,葡萄糖耐量和胰岛素耐量显着增加。此外,该组显着改变了参与肝脂质合成及其下游途径的转录调节因子。有趣的是,大多数BBP剂量与CD混合并与单独CD相比没有表型效应。雌性老鼠即使消耗了相似数量的食物,也没有表现出与雄性老鼠类似的反应。总体而言,这些数据确定了可用于BBP诱导的代谢研究模型的剂量,并表明中等剂量的EDC暴露水平可导致广泛的代谢作用。与单独的HFD相比,胰岛素耐受性差。此外,该组显着改变了参与肝脂质合成及其下游途径的转录调节因子。有趣的是,大多数BBP剂量与CD混合并与单独CD相比没有表型效应。雌性老鼠即使消耗了相似数量的食物,也没有表现出与雄性老鼠类似的反应。总体而言,这些数据确定了可用于BBP诱导的代谢研究模型的剂量,并表明中等剂量的EDC暴露水平可导致广泛的代谢作用。与单独使用HFD相比,胰岛素耐受性差。此外,该组显着改变了参与肝脂质合成及其下游途径的转录调节因子。有趣的是,大多数BBP剂量与CD混合并与单独CD相比没有表型效应。雌性老鼠即使消耗了相似数量的食物,也没有表现出与雄性老鼠类似的反应。总体而言,这些数据确定了可用于BBP诱导的代谢研究模型的剂量,并表明中等剂量的EDC暴露水平可导致广泛的代谢作用。与CD混合并与单独CD相比,大多数BBP剂量均没有表型作用。雌性老鼠即使消耗了相似数量的食物,也没有表现出与雄性老鼠类似的反应。总体而言,这些数据确定了可用于BBP诱导的代谢研究模型的剂量,并表明中等剂量的EDC暴露水平可导致广泛的代谢作用。与CD混合并与单独CD相比,大多数BBP剂量均没有表型作用。雌性老鼠即使消耗了相似数量的食物,也没有表现出与雄性老鼠类似的反应。总体而言,这些数据确定了可用于BBP诱导的代谢研究模型的剂量,并表明中等剂量的EDC暴露水平可导致广泛的代谢作用。
更新日期:2020-06-12
down
wechat
bug