Abstract

Background

A naturally occurring material, namely sporopollenin (SP), was subjected to an easy physical surface modification process called a polydopamine coating. The treatment changed the acid-base properties of the surface, so that in the new form the SP surface gained a very attractive character for anionic dyes.

Objective

The aim of the study was to develop preconcentration and subsequent spectrophotometric determination methods for two anionic colorants, brilliant blue (BB) and sunset yellow (SY), using polydopamine-coated (PDC) SP.

Method

The experiments were carried out in a column system, and the effects of experimental parameters were studied to determine optimal conditions for the quantitative, simultaneous spectrophotometric determination of the dyes.

Results

The dyes could be detected at µg/L levels in their binary mixtures, so the detection limits were found to be 1.5 and 4.3 µg/L in the linear dynamic ranges of 0.0–3.5 and 0–8 µg/mL for BB and SY, respectively. The proposed material and procedure led to quantitative recoveries of between 95 and 100% for the dyes.

Conclusions

The procedure was applied to real food samples containing BB and SY and both dyes were successfully determined in liquid and solid foodstuffs. The mussel-inspired surface modification is proposed as a useful process to modify the surface of SP.

Highlights

The mussel-inspired polydopamine dip-coating method was adopted to modify the surface of SP for the first time. The PDCSP was successfully used to create a new adsorptive preconcentration method for simultaneous spectrophotometric determination of BB and SY in foodstuffs.

中文翻译：

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新型贻贝吸附剂同时预富集和分光光度法测定某些食品中的两种色料（E110和E133）

抽象的

背景

对天然存在的材料，即孢粉（SP），进行了称为聚多巴胺涂层的简单物理表面改性工艺。这种处理改变了表面的酸碱性质，因此在新形式中，SP表面获得了对阴离子染料非常吸引人的特性。

客观的

该研究的目的是开发使用聚多巴胺涂层（PDC）SP的两种阴离子着色剂，亮蓝色（BB）和日落黄色（SY）的预浓缩和随后的分光光度测定方法。

方法

实验在色谱柱系统中进行，研究了实验参数的影响，以确定用于定量，同时分光光度法测定染料的最佳条件。

结果

染料在其二元混合物中的浓度为µg / L，因此在BB和SY的线性动态范围0.0–3.5和0–8 µg / mL中，检出限分别为1.5和4.3 µg / L，分别。所提出的材料和程序导致染料的定量回收率为95％至100％。

结论

该方法适用于含有BB和SY的真实食品样品，并且可以成功测定液体和固体食品中的两种染料。贻贝启发性的表面改性被认为是改性SP表面的有用方法。

强调

贻贝启发式聚多巴胺浸涂法首次用于SP的表面改性。PDCSP已成功用于创建一种新的吸附预浓缩方法，用于同时分光光度法测定食品中的BB和SY。