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Histological, histochemical, immunohistochemical and ultrastructural characterization of the testes of the dove
Zygote ( IF 1.5 ) Pub Date : 2020-09-03 , DOI: 10.1017/s0967199420000477
Fatma El-Zahraa A Mustafa 1 , Ruwaida Elhanbaly 1
Affiliation  

SummaryAvian testes have been used in the study of germ cell transfer, importantly for understanding the preservation and control of birds. For this purpose, we use light microscopy, electron microscopy and immunohistochemistry to understand the reproductive efficiency of dove testes. The tunica albuginea was thin and septula testes were not observed. The testicular parenchyma was formed mainly of closely packed convoluted seminiferous tubules with little interstitial area. Three types of spermatogonia were distinguished. The primary spermatocyte appeared as the largest spermatogenic cell and was identified at different stages of meiosis. Different morphological stages of the spermatid were categorized. Various cellular associations were described within the seminiferous epithelium. The cytoplasm of Sertoli cells was pale and ill defined due to its close relationship to the germinal epithelium. The spermatid attached to the luminal border of Sertoli cells and germ cells were closely associated. A single layer of myoid cells surrounded the seminiferous tubule. Testicular telocytes of doves were located in the peritubular region and near the blood vessels. Telopods appeared as long cytoplasmic processes arising from the cell body. Leydig cells were distributed singly or in small groups and cords. The intensity of androgen receptor (AR) immunostaining in the testes of the dove was established for the first time and is described in this paper.

中文翻译:

鸽子睾丸的组织学、组织化学、免疫组织化学和超微结构表征

摘要禽睾丸已用于生殖细胞转移的研究,对于了解鸟类的保存和控制很重要。为此,我们使用光学显微镜、电子显微镜和免疫组织化学来了解鸽子睾丸的繁殖效率。白膜很薄,没有观察到睾丸隔。睾丸实质主要由紧密排列的曲细精管组成,间质面积很小。区分了三种类型的精原细胞。初级精母细胞作为最大的生精细胞出现,并在减数分裂的不同阶段被鉴定。对精子细胞的不同形态阶段进行了分类。在生精上皮中描述了各种细胞关联。由于与生发上皮的密切关系,支持细胞的细胞质苍白且界限不清。附着在支持细胞和生殖细胞管腔边界上的精子细胞密切相关。单层肌样细胞围绕曲细精管。鸽子睾丸端细胞位于管周区和血管附近。端足动物表现为从细胞体产生的长细胞质突起。间质细胞单独分布或以小群和条索分布。首次确定了鸽子睾丸中雄激素受体 (AR) 免疫染色的强度,并在本文中进行了描述。单层肌样细胞围绕曲细精管。鸽子睾丸端细胞位于管周区和血管附近。端足动物表现为从细胞体产生的长细胞质突起。间质细胞单独分布或以小群和条索分布。首次确定了鸽子睾丸中雄激素受体 (AR) 免疫染色的强度,并在本文中进行了描述。单层肌样细胞围绕曲细精管。鸽子睾丸端细胞位于管周区和血管附近。端足动物表现为从细胞体产生的长细胞质突起。间质细胞单独分布或以小群和条索分布。首次确定了鸽子睾丸中雄激素受体 (AR) 免疫染色的强度,并在本文中进行了描述。
更新日期:2020-09-03
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