Abstract

Enterococcus faecalis (E. faecalis) is one of the dominant bacteria for refractory infections of teeth. Silver ions (Ag⁺) have been proved to be a strong bactericide but with high cytotoxicity and discoloration property. Simvastatin is an agent used for dyslipidemia treatment and has anti-inflammatory property. In this study, Ag⁺ and simvastatin were for the first time used in combination, and poly (lactide-co-glycolide) (PLGA) submicron particles carrying both Ag⁺ and simvastatin (AgS-PLGA) were fabricated for further investigations. Results confirmed the enhanced antibacterial activity against E. faecalis of Ag⁺ by simvastatin. AgS-PLGA could release both Ag⁺ and simvastatin for 24 days and also showed enhanced antibacterial activities. On dentin slices, AgS-PLGA could enter dentinal tubules by ultrasonic activation and inhibit the colonization of E. faecalis. AgS-PLGA showed no cytotoxicity on MC3T3-E1 cells and slight suppressive effect on RAW-264.7 cells, and could reduce the secretion of IL-6 and IL-1β of RAW-264.7 cells. AgS-PLGA could be developed as a new biomaterial for infection and inflammation control for dental and related medical treatments.

摘要

粪肠球菌（E. faecalis）是顽固性牙齿感染的主要细菌之一。银离子（Ag ⁺）已被证明是一种强杀菌剂，但具有高细胞毒性和变色特性。辛伐他汀是一种用于治疗血脂异常的药物，具有抗炎特性。在这项研究中，Ag ⁺和辛伐他汀首次联合使用，并制备了携带 Ag ⁺和辛伐他汀 (AgS-PLGA) 的聚（丙交酯-共-乙交酯）（PLGA）亚微米颗粒以进行进一步研究。结果证实了辛伐他汀对Ag ⁺对粪肠球菌的抗菌活性增强。AgS-PLGA 可以同时释放 Ag ⁺和辛伐他汀 24 天，也显示出增强的抗菌活性。在牙本质切片上，AgS-PLGA 可以通过超声波激活进入牙本质小管，抑制粪肠球菌的定植。AgS-PLGA对MC3T3-E1细胞无细胞毒性，对RAW-264.7细胞有轻微抑制作用，可降低RAW-264.7细胞IL-6和IL-1β的分泌。AgS-PLGA 可以开发为一种新的生物材料，用于牙科和相关医学治疗的感染和炎症控制。