Abstract

By applying the mixed-ligand method, a new Co(II)-based coordination polymer with the chemical formula of {[Co₂L(4,4′-bipy)_0.5(H₂O)] · 0.25H₂O}_n (1) have been successfully prepared by reaction of Co(NO₃)₂ · 6H₂O and (benzylazanediyl)bis(methylene)-diphosphonic acid (L) with the presence of a second N-donor ligand 4,4′-bipyridine (4,4′-bipy). The treatment activity of the compound on the fracture was evaluated and the related mechanism was explored at the same time. First, the real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR) was performed to determine the relative expression levels of the cd133 in the fracture animal model after compound treatment. In addition to this, the western blotting assay was performed and the activation levels of the wnt signaling pathway in the mesenchymal stem cells was measured as well. Both nitrogen and oxygen atoms on the complex are found to be able to form interactions with target protein.

摘要

通过应用混合配体法，一种新的Co(II)基配位聚合物，其化学式为{[Co ₂ L(4,4'-bipy) _0.5 (H ₂ O)] · 0.25H ₂ O} _n ( 1 ) Co(NO ₃ ) ₂  · 6H ₂反应成功制备O 和（苄基氮杂二基）双（亚甲基）-二膦酸（L），存在第二个 N 供体配体 4,4'-联吡啶（4,4'-bipy）。评价了该化合物对骨折的治疗活性，同时探索了相关机制。首先，进行实时逆转录聚合酶链反应（RT-PCR）以确定复合治疗后骨折动物模型中cd133的相对表达水平。除此之外，还进行了蛋白质印迹分析，并测量了间充质干细胞中 wnt 信号通路的激活水平。发现复合物上的氮和氧原子都能够与目标蛋白质形成相互作用。