Abstract

The present work was conducted to biofabricate silver nanoparticles (AgNPs) in single step employing aqueous extract of Dregea volubilis flowers and to investigate its antioxidant, antidiabetic and antibacterial activities. The AgNPs were characterized by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray (EDX) spectrometry, high resolution transmission electron microscopy (HRTEM) and zeta potential study. The biofabricated AgNPs showed potential antioxidant activity for scavenging DPPH radical (IC₅₀, 40.45 ± 5.06 μg/mL), ABTS radical (IC₅₀, 78.49 ± 1.41 μg/mL), total antioxidant capacity (148.83 ± 2.99 mg GAE/g) along with remarkable inhibitory effects on α-amylase (IC₅₀, 10.62 ± 0.22 μg/mL) and α-glucosidase (IC₅₀, 6.49 ± 0.03 μg/mL). The AgNPs showed notable antibacterial activity in terms of zone of inhibition (in mm) against Pseudomonas aeruginosa (10.67 ± 0.44), Escherichia coli (9.33 ± 0.44), Bacillus subtilis (14.67 ± 0.60) and Staphylococcus aureus (15.67 ± 0.60).

摘要

目前的工作是使用Dregea volubilis花的水提取物一步生物制造银纳米粒子 (AgNPs)，并研究其抗氧化、抗糖尿病和抗菌活性。AgNPs 通过紫外-可见光谱、X 射线衍射 (XRD)、傅里叶变换红外光谱 (FTIR)、场发射扫描电子显微镜 (FESEM)、能量色散 X 射线 (EDX) 光谱、高分辨率透射电子显微镜 (HRTEM) 和 zeta 电位研究。生物制造的 AgNPs 在清除 DPPH 自由基 (IC ₅₀ , 40.45 ± 5.06 μg/mL)、ABTS 自由基 (IC ₅₀, 78.49 ± 1.41 μg/mL)、总抗氧化能力 (148.83 ± 2.99 mg GAE/g) 以及对 α-淀粉酶 (IC ₅₀ , 10.62 ± 0.22 μg/mL) 和 α-葡萄糖苷酶 (IC ₅₀ , 6. ± 0.03 微克/毫升）。AgNPs 对铜绿假单胞菌(10.67 ± 0.44) 、大肠杆菌(9.33 ± 0.44)、枯草芽孢杆菌(14.67 ± 0.60) 和金黄色葡萄球菌( 15.60) 的抑制区 (mm) 显示出显着的抗菌活性。