Abstract

Identification of new modifications and the association with diet patterns are essential for the prevention of non-alcoholic fatty liver disease (NAFLD). To address this problem, we feed rats with high caloric diets based on high sucrose (HSD) and high fat (HFD) and analysed metabolic and mitochondrial alterations. Both diets induce moderated obesity and fat accumulation in the liver after 8, 10 and 12 months of diet. The HSD induces both hyperleptinemia and hyperinsulinemia, as well as up-regulation of transcription factors SRBEP1 and PPARγ along slight increase nitrosylation of proteins and increased mitochondrial fission. In contrast, HFD induced hyperleptinemia without changes in neither insulin levels nor oxidative stress, SREBP1, PPARγ, or mitochondrial dynamics. In conclusion, chronic consumption of high sucrose content diets induces more pathological and metabolic alteration in liver in comparison with consumption of high-fat content diets, although both induces obesity and liver steatosis in these animal models.

摘要

识别新的修饰以及与饮食模式的关联对于预防非酒精性脂肪肝疾病 (NAFLD) 至关重要。为了解决这个问题，我们用基于高蔗糖 (HSD) 和高脂肪 (HFD) 的高热量饮食喂养大鼠，并分析了代谢和线粒体的变化。在饮食 8、10 和 12 个月后，这两种饮食都会导致适度肥胖和肝脏脂肪堆积。HSD 诱导高瘦素血症和高胰岛素血症，以及转录因子 SRBEP1 和 PPARγ 的上调以及蛋白质亚硝基化的轻微增加和线粒体分裂的增加。相比之下，HFD 诱发高瘦素血症时胰岛素水平和氧化应激、SREBP1、PPARγ 或线粒体动力学均未发生变化。综上所述，